Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii
Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii
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DOI：
10.1021/acssynbio.8b00251
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发表时间：
2018-09-01
影响因子：
4.7
通讯作者：
Lemaire, Stephane D.
中科院分区：
文献类型：
--
作者：
Crozet, Pierre;Navarro, Francisco J.;Lemaire, Stephane D.
微藻被认为是有前途的生物体，可以根据其光合能力开发创新概念，比异养宿主提供更可持续的生产。然而，要实现其作为绿色细胞工厂的潜力，一个主要挑战是使微藻更容易设计。快速且可预测的基因操作的一种有前途的方法是使用标准化的合成生物学工具和工作流程。为此，我们开发了针对绿色微藻莱茵衣藻的模块化克隆工具包。它基于采用标准语法的 Golden Gate 克隆，包含 119 个公开分布的遗传部分，其中大部分已在多个菌株中进行了功能验证。它包含启动子、UTR、终止子、标签、报告基因、抗生素抗性基因和克隆在不同位置的内含子，以实现最大程度的模块化。该工具包能够快速构建用于基础研究和藻类生物技术的工程细胞。这项工作将使衣藻成为可持续合成生物学的下一个基础。
Microalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, to realize their potential as green cell factories, a major challenge is to make microalgae easier to engineer. A promising approach for rapid and predictable genetic manipulation is to use standardized synthetic biology tools and workflows. To this end we have developed a Modular Cloning toolkit for the green microalga Chlamydomonas reinhardtii. It is based on Golden Gate cloning with standard syntax, and comprises 119 openly distributed genetic parts, most of which have been functionally validated in several strains. It contains promoters, UTRs, terminators, tags, reporters, antibiotic resistance genes, and introns cloned in various positions to allow maximum modularity. The toolkit enables rapid building of engineered cells for both fundamental research and algal biotechnology. This work will make Chlamydomonas the next chassis for sustainable synthetic biology.