Adsorption Force of Fibronectin on Various Surface Chemistries and Its Vital Role in Osteoblast Adhesion
Adsorption Force of Fibronectin on Various Surface Chemistries and Its Vital Role in Osteoblast Adhesion
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纤连蛋白对各种表面化学物质的吸附力及其在成骨细胞粘附中的重要作用。
DOI：
10.1021/bm501873g
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发表时间：
2015-03-01
影响因子：
6.2
通讯作者：
Luo, Yanfeng
中科院分区：
文献类型：
--
作者：
Lin, Manping;Wang, Huaiyu;Luo, Yanfeng
吸附在植入生物材料上的蛋白质的量、类型和构象被认为影响细胞粘附。然而，只有少数研究工作致力于蛋白质吸附力的贡献。为了验证蛋白质在生物材料上的吸附力是细胞粘附的另一个重要介质的假设，利用球/平面吸附模型和平行板流动室，定量了纤维连接蛋白（FN）在末端为−OH、− CH 3和− NH 2的自组装单分子膜（SAMs）上的吸附力（Fad）.正如所揭示的，Fadon自组装膜遵循− NH 2> − CH 3 <$−OH的化学依赖性。进一步证明Fad与FN构象共同调节成骨细胞的晚期粘附以及随之而来的吸附FN的重组和内源性FN的纤维形成。我们的研究表明，蛋白质吸附力在细胞粘附中起着关键作用，应该参与更好的生物材料设计。
The amount, type, and conformation of proteins adsorbed on an implanted biomaterial are believed to influence cell adhesion. Nevertheless, only a few research works have been dedicated to the contribution of protein adsorption force. To verify our hypothesis that the adsorption force of protein on biomaterial is another crucial mediator to cell adhesion, fibronectin (FN) adsorbed on self-assembled monolayers (SAMs) with terminal −OH, −CH3, and −NH2was quantified for FN adsorption force (Fad) by utilizing a sphere/plane adsorption model and parallel plate flow chamber. As revealed,Fadon SAMs followed a chemistry-dependence of −NH2> −CH3≫ −OH. It is further demonstrated thatFadtogether with FN conformation could regulate the late osteoblast adhesion and the consequent reorganization of the adsorbed FN and fibrillogenesis of the endogenous FN. Our study suggests that protein adsorption force plays a key role in cell adhesion and should be involved for better biomaterial design.