A Defined-Multispecies Microbial Model for Studying Enamel Caries Development
A Defined-Multispecies Microbial Model for Studying Enamel Caries Development
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DOI：
10.1159/000347050
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发表时间：
2013-01-01
期刊：
影响因子：
4.2
通讯作者：
Zero, D. T.
中科院分区：
文献类型：
--
作者：
Arthur, R. A.;Waeiss, R. A.;Zero, D. T.
本研究的目的是描述和验证龋齿发展的体外多物种微生物生物膜模型，通过评估不同介质浓度的蔗糖（0.5和1.0%）和氟化物（0.4，0.8和1.0 ppm F）在研究1中，和钙（1.0和2.0 mm Ca）在研究2中的影响。干酪乳杆菌、变形链球菌、S. salivarius和S. sanguinis在具有已知基线表面硬度的唾液膜涂覆的釉质板的表面上生长;每天更换生长培养基。生物膜上的活细胞计数和表面显微硬度变化的百分比（%SMC），损伤深度（LD）和综合矿物质损失（IML）的生物膜形成4天后，在测试条件下的釉质板进行了评估。蔗糖、氟和钙浓度对生物膜上的活细胞数有显著影响（p < 0.05）。随着氟和钙浓度的增加，%SMC降低（p < 0.001）。在0.8和1.0 ppm F存在下，IML（p < 0.001）和LD（p < 0.05）较低。反应变量（%SMC，LD和IML）与氟和钙浓度之间呈负相关。结果表明，开发的微生物龋齿模型能够显示不同水平的龋齿抑制响应氟和钙浓度，证实了临床观察。蔗糖浓度对龋齿发展的影响，发现只有在存在的最低氟浓度。版权所有（C）2013 S. Karger AG，巴塞尔
The aims of this study were to describe and validate an in vitro multispecies microbial biofilm model for caries development by evaluating the effects of varying medium concentration of sucrose (0.5 and 1.0%) and fluoride (0.4, 0.8 and 1.0 ppm F) in study 1, and calcium (1.0 and 2.0 mm Ca) in study 2. Defined-multispecies biofilms, formed by Lactobacillus casei, Streptococcus mutans, S. salivarius and S. sanguinis, were grown on the surface of salivary-pellicle-coated enamel slabs, with known baseline surface hardness; growth medium was changed daily. Counts of viable cells on biofilms and the percentage of surface microhardness change (%SMC), lesion depth (LD) and integrated mineral loss (IML) on enamel slabs were assessed after 4 days of biofilm formation under the tested conditions. Counts of viable cells on biofilms were significantly affected by sucrose, fluoride and calcium concentrations (p < 0.05). There was a decrease in %SMC in response to increased fluoride and calcium concentrations (p < 0.001). Lower IML (p < 0.001) and LD (p < 0.05) were found in the presence of 0.8 and 1.0 ppm F. A negative correlation was found between the response variables (%SMC, LD and IML) and fluoride and calcium concentrations. The results suggest that the microbial caries model developed was able to show distinct levels of caries inhibition in response to fluoride and calcium concentrations, corroborating clinical observations. An effect of sucrose concentration on caries development was found only in the presence of the lowest fluoride concentration. Copyright (C) 2013 S. Karger AG, Basel