Fluorescent proteins as biomarkers and biosensors: throwing color lights on molecular and cellular processes.
Fluorescent proteins as biomarkers and biosensors: throwing color lights on molecular and cellular processes.
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DOI：
10.2174/138920308785132668
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发表时间：
2008-08
影响因子：
2.8
通讯作者：
Turoverov KK
中科院分区：
文献类型：
--
作者：
Stepanenko OV;Verkhusha VV;Kuznetsova IM;Uversky VN;Turoverov KK
来自维多利亚多管发光水母的绿色荧光蛋白（GFP）是细胞生物学中研究最广泛、应用最广泛的蛋白。 GFP 样蛋白构成了一个快速增长的家族，因为已经发现了几种天然存在的 GFP 样蛋白，并且已经创建了水母 GFP 的增强突变体。这些突变体在构象稳定性、量子产率、光谱特性（吸收和荧光光谱的位置）和光化学特性方面与野生型 GFP 不同。类 GFP 蛋白非常多样化，因为它们不仅可以是绿色，还可以是蓝色、橙红色、远红色、青色和黄色。它们还可以具有双色荧光（例如绿色和红色）或非荧光。其中一些具有点燃特性，一些具有光激活特性，一些具有光开关特性。本综述试图描述类 GFP 蛋白的主要颜色组，描述其结构和生色团形成机制，系统化其构象稳定性数据，并总结其在细胞和分子生物学中用作标记和生物传感器的主要趋势。
Green fluorescent protein (GFP) from jellyfish Aequorea victoria is the most extensively studied and widely used in cell biology protein. GFP-like proteins constitute a fast growing family as several naturally occurring GFP-like proteins have been discovered and enhanced mutants of Aequorea GFP have been created. These mutants differ from wild-type GFP by conformational stability, quantum yield, spectroscopic properties (positions of absorption and fluorescence spectra) and by photochemical properties. GFP-like proteins are very diverse, as they can be not only green, but also blue, orange-red, far-red, cyan, and yellow. They also can have dual-color fluorescence (e.g., green and red) or be non-fluorescent. Some of them possess kindling property, some are photoactivatable, and some are photoswitchable. This review is an attempt to characterize the main color groups of GFP-like proteins, describe their structure and mechanisms of chromophore formation, systemize data on their conformational stability and summarize the main trends of their utilization as markers and biosensors in cell and molecular biology.
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DOI：
10.1073/pnas.97.22.11984
发表时间：
2000-10-24
影响因子：
11.1
作者：
Baird, GS;Zacharias, DA;Tsien, RY
通讯作者：
Tsien, RY
影响因子：
46.9
作者：
Chudakov, DM;Belousov, VV;Lukyanov, KA
通讯作者：
Lukyanov, KA
DOI：
10.1073/pnas.96.7.3590
发表时间：
1999-03-30
影响因子：
11.1
作者：
Chiti, F;Webster, P;Dobson, CM
通讯作者：
Dobson, CM
影响因子：
3.5
作者：
Bloemberg, GV;Wijfjes, AHM;Lugtenberg, BJJ
通讯作者：
Lugtenberg, BJJ
影响因子：
46.9
作者：
Cabantous, S;Terwilliger, TC;Waldo, GS
通讯作者：
Waldo, GS