Structural definition of a potent macrophage activating factor derived from vitamin D3-binding protein with adjuvant activity for antibody production.
Structural definition of a potent macrophage activating factor derived from vitamin D3-binding protein with adjuvant activity for antibody production.
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源自维生素 D3 结合蛋白的强效巨噬细胞激活因子的结构定义，具有抗体生成的佐剂活性。
DOI：
10.1016/s0161-5890(96)00081-8
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发表时间：
1996
影响因子：
3.6
通讯作者：
Yamamoto,N
中科院分区：
文献类型：
--
作者：
Yamamoto,N
将人维生素D3结合蛋白（Gc蛋白）与固定化β-半乳糖苷酶和唾液酸酶的混合物孵育，有效地产生了一种有效的巨噬细胞活化因子，一种以N-乙酰半乳糖胺作为剩余糖的蛋白质。将Gc蛋白与固定化β-半乳糖苷酶和唾液酸酶逐步孵育，并用固定化凝集素分离中间产物，表明这些糖苷酶水解Gc糖蛋白的任一顺序都产生巨噬细胞活化因子，这意味着Gc蛋白携带由N-乙酰半乳糖胺和二支链半乳糖和唾液酸末端组成的三糖。小鼠腹腔巨噬细胞与皮摩尔量的酶促产生的巨噬细胞活化因子（GcMAF）孵育3小时，导致吞噬活性大大增强。给予微量（10-50 pg/小鼠）的GcMAF导致巨噬细胞的吞噬活性增强7 - 9倍。将绵羊红细胞（SRBC）沿着GcMAF注射到小鼠体内，在2-4天内产生大量分泌抗SRBC抗体的脾细胞。
Incubation of human vitamin D3-binding protein (Gc protein), with a mixture of immobilized β-galactosidase and sialidase, efficiently generated a potent macrophage activating factor, a protein with N-acetylgalactosamine as the remaining sugar. Stepwise incubation of Gc protein with immobilized β-galactosidase and sialidase, and isolation of the intermediates with immobilized lectins, revealed that either sequence of hydrolysis of Gc glycoprotein by these glycosidases yields the macrophage-activating factor, implying that Gc protein carries a trisaccharide composed of N-acetylgalactosamine and dibranched galactose and sialic acid termini. A 3 hr incubation of mouse peritoneal macrophages with picomolar amounts of the enzymatically generated macrophage-activating factor (GcMAF) resulted in a greatly enhanced phagocytic activity. Administration of a minute amount (10–50 pg/mouse) of GcMAF resulted in a seven- to nine-fold enhanced phagocytic activity of macrophages. Injection of sheep red blood cells (SRBC) along with GcMAF into mice produced a large number of anti-SRBC antibody secreting splenic cells in 2–4 days.