Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd
Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd
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DOI：
10.1128/jb.01422-06
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发表时间：
2007-03-01
影响因子：
3.2
通讯作者：
Inzana, Thomas J.
中科院分区：
文献类型：
--
作者：
Challacombe, Jean F.;Duncan, A. J.;Inzana, Thomas J.
睡眠嗜血杆菌可以是牛粘膜表面的共生菌，也可以是机会致病菌。 H. sontnus 致病菌株是牛全身性疾病的重要原因。我们报告了睡眠嗜血菌 129Pt（一种非致病性共生包皮分离株）的基因组序列，以及睡眠嗜血菌 129Pt、流感嗜血杆菌 Rd 和杜克雷嗜血杆菌 35000HP 的全基因组比较分析结果。我们在 H. somnus 129Pt 中发现了涉及脂寡糖生物合成、碳水化合物摄取和代谢、阳离子转运、氨基酸代谢、泛醌和甲基萘醌生物合成、细胞表面粘附、辅因子生物合成、能量代谢和电子传递的独特基因。这三种生物体之间也有许多共同的基因。我们对睡眠嗜血杆菌 129Pt、流感嗜血杆菌 Rd 和杜克雷嗜血杆菌 35000HP 的比较分析揭示了参与代谢、宿主定植和持久性的基因数量和组成的相似性和差异。这些结果为研究这些生物的宿主特异性和生态位偏好奠定了基础。未来 H. sontnus 129Pt 和强毒株之间的比较将有助于制定保护策略和疫苗，以保护牛免受 H. somnus 疾病的侵害。
Haemophilus somnus can be either a commensal of bovine mucosal surfaces or an opportunistic pathogen. Pathogenic strains of H. sontnus are a significant cause of systemic disease in cattle. We report the genome sequence of H. somnus 129Pt, a nonpathogenic commensal preputial isolate, and the results of a genome-wide comparative analysis of H. somnus 129Pt, Haemophilus influenzae Rd, and Haemophilus ducreyi 35000HP. We found unique genes in H. somnus 129Pt involved in lipooligosaccharide biosynthesis, carbohydrate uptake and metabolism, cation transport, amino acid metabolism, ubiquinone and menaquinone biosynthesis, cell surface adhesion, biosynthesis of cofactors, energy metabolism, and electron transport. There were also many genes in common among the three organisms. Our comparative analyses of H. somnus 129Pt, H. influenzae Rd, and H. ducreyi 35000HP revealed similarities and differences in the numbers and compositions of genes involved in metabolism, host colonization, and persistence. These results lay a foundation for research on the host specificities and niche preferences of these organisms. Future comparisons between H. sontnus 129Pt and virulent strains will aid in the development of protective strategies and vaccines to protect cattle against H. somnus disease.