INVESTIGATION OF THE IGE-BINDING PROTEINS IN SOYBEANS BY IMMUNOBLOTTING WITH THE SERA OF THE SOYBEAN-SENSITIVE PATIENTS WITH ATOPIC-DERMATITIS
INVESTIGATION OF THE IGE-BINDING PROTEINS IN SOYBEANS BY IMMUNOBLOTTING WITH THE SERA OF THE SOYBEAN-SENSITIVE PATIENTS WITH ATOPIC-DERMATITIS
复制标题
DOI：
10.3177/jnsv.37.555
复制
发表时间：
1991-12-01
影响因子：
1.6
通讯作者：
SASAOKA, K
中科院分区：
文献类型：
--
作者：
OGAWA, T;BANDO, N;SASAOKA, K
大豆中的 IgE 结合蛋白通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对用特应性皮炎患者血清探查的分级大豆蛋白进行表征。大约 20% 的接受检查的患者体内显示出针对大豆蛋白的特异性 IgE 抗体。患者血清中至少识别出 16 种分子量范围为 70,000 至 14,000 的大豆蛋白：其中 10 种主要 IgE 结合成分存在于 7S 球蛋白组分中，其他主要存在于 2S 球蛋白和乳清组分中。患者的 IgE 抗体与 7S-球蛋白组分中分子量约为 30,000 的独特蛋白质结合最强烈且最频繁，该蛋白质似乎是大豆中的主要过敏原，被命名为 Gly m Bd 30 K。11S-球蛋白组分中的蛋白质几乎不被患者血清识别，并被认为对特应性皮炎患者的过敏性较低。
The IgE-binding proteins in soybeans were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the fractionated soybean proteins probed with the sera of the patients with atopic dermatitis. About 20% of the patients examined were shown to have specific IgE antibodies to soybean proteins. At least 16 soybean proteins with molecular weights ranging from about 70,000 to 14,000 were recognized by the sera of the patients: 10 major IgE-binding components were found in the 7S-globulin fraction, and the others mainly in the 2S-globulin and whey fractions. The IgE antibodies of the patients bound most strongly and frequently to a unique protein with molecular weight of about 30,000 in the 7S-globulin fraction, which appeared to be the major allergen in soybeans and was named as Gly m Bd 30 K. The proteins in the 11S-globulin fraction were scarcely recognized by the patients' sera and assumed to be less allergenic for the patients with atopic dermatitis.