Quantification of noise sources for amperometric measurement of quantal exocytosis using microelectrodes.
Quantification of noise sources for amperometric measurement of quantal exocytosis using microelectrodes.
复制标题
使用微电极对量子胞吐作用的电流测量的噪声源进行量化。
DOI：
10.1039/c2an35157a
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Gillis,KevinD
中科院分区：
文献类型：
--
作者：
Yao,Jia;Gillis,KevinD
电化学微电极通常用于记录电流的安培尖峰，其由胞吐期间从单个囊泡释放的递质的氧化引起。虽然这些测量的灵敏度非常高，但需要更好地了解限制该技术分辨率的噪声源，以指导下一代器件的设计。我们测量了电化学微电极的电流功率谱密度（SI），以了解主要噪声源的物理基础，并确定噪声如何随电极材料和几何形状而变化。我们发现，电流噪声是热的起源，因为SI是正比于电极的导纳的真实的部分。微电极的导纳可以用一个恒相单元模型来描述，使得真实的和虚导纳都随频率的增加而增加，其幂次为0.84-0.96。结果表明，电流标准偏差与工作电极面积的平方根成正比，随记录带宽呈线性增加，并随电极材料的选择而变化，Au-碳纤维>掺氮类金刚石碳>氧化铟锡。细胞和微电极之间的接触不会明显增加噪声。表面图案化的微芯片电极可以具有优于相同面积的碳纤维微电极的上级噪声性能。
Electrochemical microelectrodes are commonly used to record amperometric spikes of current that result from oxidation of transmitter released from individual vesicles during exocytosis. Whereas the exquisite sensitivity of these measurements is well appreciated, a better understanding of the noise sources that limit the resolution of the technique is needed to guide the design of next-generation devices. We measured the current power spectral density (SI) of electrochemical microelectrodes to understand the physical basis of dominant noise sources and to determine how noise varies with the electrode material and geometry. We find that the current noise is thermal in origin in that SI is proportional to the real part of the admittance of the electrode. The admittance of microelectrodes is well described by a constant phase element model such that both the real and imaginary admittance increase with frequency raised to a power of 0.84–0.96. Our results demonstrate that the current standard deviation is proportional to the square root of the area of the working electrode, increases ∼linearly with the bandwidth of the recording, and varies with the choice of the electrode material with Au ≈ carbon fiber > nitrogen-doped diamond-like carbon > indium-tin-oxide. Contact between a cell and a microelectrode does not appreciably increase noise. Surface-patterned microchip electrodes can have a noise performance that is superior to that of carbon-fiber microelectrodes of the same area.
影响因子：
10.6
作者：
PEROUTKA, SJ
通讯作者：
PEROUTKA, SJ
豚鼠和大鼠海马膜中 5-羟色胺 1a 受体介导的毛喉素刺激的腺苷酸环化酶活性抑制作用的表征。
DOI：
--
发表时间：
1986
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
DeVivo,M;Maayani,S
通讯作者：
Maayani,S