Meta-analysis of amino acid stable nitrogen isotope ratios for estimating trophic position in marine organisms
Meta-analysis of amino acid stable nitrogen isotope ratios for estimating trophic position in marine organisms
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DOI：
10.1007/s00442-015-3305-7
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发表时间：
2015-07-01
期刊：
影响因子：
2.7
通讯作者：
Winder, Monika
中科院分区：
文献类型：
--
作者：
Nielsen, Jens M.;Popp, Brian N.;Winder, Monika
在复杂的生态系统中，营养结构的估计是一种常用的方法，用于检索有关能量途径，捕食和竞争的信息。氨基酸（AA）化合物氮（N）同位素分析（CSIA）是一种较新的生物营养位置（TP）和摄食关系估计方法。在这里，我们进行了第一次荟萃分析三角洲N-15 AA值从测量的359个海洋物种，涵盖四个营养级，并比较TP估计AA-CSIA文献值来自食品，肠道或胃内容物分析。我们测试了AA营养富集因子（TEF）或不同个体AA之间的N-15富集是否在营养水平上是恒定的，以及是否包含来自多个AA的Δ N-15值可以改善TP估计。对于谷氨酸相对于苯丙氨酸（Phe）的TEF，我们发现所有分类群的平均值为6.6 aEuro度，显著低于通常应用的7.6 aEuro度。我们发现，有机体饲养生态影响TEF值的几个营养AA相对于苯丙氨酸，与食草动物的TEF值显着较高的杂食动物和食肉动物相比，而TEF值也显着较低的动物排泄尿素相比，铵。基于使用Δ N-15 AA值的元数据的多个模型结构的比较，我们表明，原则上增加AA的数量提高了TP估计的精度。该荟萃分析阐明了使用个体Δ N-15 AA值作为营养生态学工具的优点和局限性，并为AA-CSIA在食物网研究中的未来应用提供了指导。
Estimating trophic structures is a common approach used to retrieve information regarding energy pathways, predation, and competition in complex ecosystems. The application of amino acid (AA) compound-specific nitrogen (N) isotope analysis (CSIA) is a relatively new method used to estimate trophic position (TP) and feeding relationships in diverse organisms. Here, we conducted the first meta-analysis of delta N-15 AA values from measurements of 359 marine species covering four trophic levels, and compared TP estimates from AA-CSIA to literature values derived from food items, gut or stomach content analysis. We tested whether the AA trophic enrichment factor (TEF), or the N-15 enrichment among different individual AAs is constant across trophic levels and whether inclusion of delta N-15 values from multiple AAs improves TP estimation. For the TEF of glutamic acid relative to phenylalanine (Phe) we found an average value of 6.6 aEuro degrees across all taxa, which is significantly lower than the commonly applied 7.6 aEuro degrees. We found that organism feeding ecology influences TEF values of several trophic AAs relative to Phe, with significantly higher TEF values for herbivores compared to omnivores and carnivores, while TEF values were also significantly lower for animals excreting urea compared to ammonium. Based on the comparison of multiple model structures using the metadata of delta N-15 AA values we show that increasing the number of AAs in principle improves precision in TP estimation. This meta-analysis clarifies the advantages and limitations of using individual delta N-15 AA values as tools in trophic ecology and provides a guideline for the future application of AA-CSIA to food web studies.