Structure and regulation of OPR1 and OPR2, two closely related genes encoding 12-oxophytodienoic acid-10,11-reductases from Arabidopsis thaliana
Structure and regulation of OPR1 and OPR2, two closely related genes encoding 12-oxophytodienoic acid-10,11-reductases from Arabidopsis thaliana
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DOI：
10.1007/s004250050545
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发表时间：
1999-04-01
期刊：
影响因子：
4.3
通讯作者：
Weiler, EW
中科院分区：
文献类型：
--
作者：
Biesgen, C;Weiler, EW
从拟南芥（Arabidopsis thaliana（L.））基因组中克隆了两个12-氧代植物二烯酸还原酶1.3.1.42嘿这两个基因的组织进行了测定，并确定了推定的顺式元件。在转基因拟南芥和烟草中表达的启动子-β-葡萄糖醛酸酶（GUS）融合体。植物揭示了在花中OPR启动子驱动的GUS表达的差异。而OPR 1启动子指导GUS在幼嫩种子中的表达，OPR 2启动子指导花粉特异性表达。OPR 1和OPR 2主要在根中表达。应激处理，如局部和全身创伤，UV-C照射和寒冷，导致稳态OPR mRNA水平的瞬时变化，但没有检测到多肽水平或酶活性的同时变化。
The genes of two closely related 12-oxophytodienoic acid reductases (EC 1.3.1.42), OPR1 and OPR2, were identified on a 7079-bp-long genomic fragment from Arabidopsis thaliana (L.) Heynh. The organization of these two genes was determined and putative cis elements were identified. Promoter-beta-glucuronidase (GUS) fusions expressed in transgenic Arabidopsis thaliana and Nicotiana tabacum L. plants revealed differences in OPR-promoter-driven GUS expression in flowers. While the OPR1 promoter directed GUS expression in young seeds, the OPR2 promoter directed pollen-specific expression. Both OPR1 and OPR2, were predominantly expressed in roots. Stress treatments, like local and systemic wounding, UV-C illumination and coldness, resulted in transient changes in steady-state OPR mRNA levels, but no concurrent changes in polypeptide level or enzyme activity were detected.