RNA-DNA hybrid nanoshapes that self-assemble dependent on ligand binding
RNA-DNA hybrid nanoshapes that self-assemble dependent on ligand binding
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DOI：
10.1039/c9nr09706f
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发表时间：
2020-02-07
期刊：
影响因子：
6.7
通讯作者：
Hermann, Thomas
中科院分区：
文献类型：
--
作者：
Chen, Shi;Hermann, Thomas
核酸纳米结构的自组装是通过互补序列之间的碱基配对通过寡核苷酸模块的选择性缔合来驱动的。在此，我们报告了在腺苷配体的控制下有条件地组装的RNA-DNA混合纳米形状的发展。纳米形状的设计概念依赖于DNA适体的配体依赖性稳定化，DNA适体作为边缘稳定的RNA角模块之间的连接器。配体依赖性RNA-DNA纳米形状通过将腺苷结合偶联到环状闭合结构的形成而在全有或全无的过程中自组装，所述环状闭合结构通过在所得多边形中的连续碱基堆叠而稳定。通过筛选各种DNA适体构建体与RNA角模块的组合以形成稳定的复合物，我们确定了腺苷依赖性纳米方，其形状通过原子力显微镜确认。作为传感器应用的概念验证，通过DNA适体组分的染料缀合获得腺苷响应性FRET活性纳米方。
Self-assembly of nucleic acid nanostructures is driven by selective association of oligonucleotide modules through base pairing between complementary sequences. Herein, we report the development of RNA-DNA hybrid nanoshapes that conditionally assemble under the control of an adenosine ligand. The design concept for the nanoshapes relies on ligand-dependent stabilization of DNA aptamers that serve as connectors between marginally stable RNA corner modules. Ligand-dependent RNA-DNA nanoshapes self-assemble in an all-or-nothing process by coupling adenosine binding to the formation of circularly closed structures which are stabilized through continuous base stacking in the resulting polygons. By screening combinations of various DNA aptamer constructs with RNA corner modules for the formation of stable complexes, we identified adenosine-dependent nanosquares whose shape was confirmed by atomic force microscopy. As a proof-of-concept for sensor applications, adenosine-responsive FRET-active nanosquares were obtained by dye conjugation of the DNA aptamer components.