Transitional flow at the venous anastomosis of an arteriovenous graft: potential activation of the ERK1/2 mechanotransduction pathway.
Transitional flow at the venous anastomosis of an arteriovenous graft: potential activation of the ERK1/2 mechanotransduction pathway.
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动静脉移植物静脉吻合处的过渡血流：ERK1/2 机械转导途径的潜在激活。
DOI：
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
H. Bassiouny
中科院分区：
文献类型：
--
作者：
F. Loth;P. Fischer;N. Arslan;C. Bertram;S. Lee;T. Royston;Wael E Shaalan;H. Bassiouny
我们目前的实验和计算结果，描述的水平，分布和重要性的动静脉移植静脉吻合内的速度波动。这项工作的动机是为了更好地了解生物力学力量在这些移植物内内膜增生的发展中的重要性。在代表静脉吻合的移植物模型内进行了体外稳流研究（Re = 1060和1820），以通过激光多普勒风速仪测量速度。采用谱元技术对相同几何条件和流动条件下的流动进行了数值模拟。当血流从移植物进入静脉时，速度场表现出源于分离剪切层的流动分离和相干结构（弱湍流）。我们还报告了猪动物研究的结果，在移植物构建时测量静脉吻合口静脉壁振动的分布和幅度。初步的分子生物学研究表明，在静脉壁振动增加的区域，细胞外调节激酶ERK 1/2（一种参与机械转导的促分裂原活化蛋白激酶）的活性水平升高。这些发现表明，相关的药物诱导的静脉壁振动和动静脉移植物内膜增生的发展之间存在潜在的关系。然而，为了确定是否存在相关性，并区分振动效应与流动相关效应，还需要进一步研究。
We present experimental and computational results that describe the level, distribution, and importance of velocity fluctuations within the venous anastomosis of an arteriovenous graft. The motivation of this work is to understand better the importance of biomechanical forces in the development of intimal hyperplasia within these grafts. Steady-flow in vitro studies (Re = 1060 and 1820) were conducted within a graft model that represents the venous anastomosis to measure velocity by means of laser Doppler anemometry. Numerical simulations with the same geometry and flow conditions were conducted by employing the spectral element technique. As flow enters the vein from the graft, the velocity field exhibits flow separation and coherent structures (weak turbulence) that originate from the separation shear layer. We also report results of a porcine animal study in which the distribution and magnitude of vein-wall vibration on the venous anastomosis were measured at the time of graft construction. Preliminary molecular biology studies indicate elevated activity levels of the extracellular regulatory kinase ERK1/2, a mitogen-activated protein kinase involved in mechanotransduction, at regions of increased vein-wall vibration. These findings suggest a potential relationship between the associated turbulence-induced vein-wall vibration and the development of intimal hyperplasia in arteriovenous grafts. Further research is necessary, however, in order to determine if a correlation exists and to differentiate the vibration effect from that of flow related effects.
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DOI：
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发表时间：
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