SLC7 amino acid transporters of the yellow fever mosquito Aedes aegypti and their role in fat body TOR signaling and reproduction.
SLC7 amino acid transporters of the yellow fever mosquito Aedes aegypti and their role in fat body TOR signaling and reproduction.
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DOI：
10.1016/j.jinsphys.2012.01.005
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发表时间：
2012-04
影响因子：
2.2
通讯作者：
Hansen IA
中科院分区：
文献类型：
--
作者：
Carpenter VK;Drake LL;Aguirre SE;Price DP;Rodriguez SD;Hansen IA
成年雌蚊脂肪体的一个重要功能是将血粉衍生的氨基酸（AA）转化为大量的卵黄蛋白前体。AA穿过脂肪体滋养细胞的质膜的高效运输机制对于递送用于快速合成大量这些蛋白质的构件是必不可少的。该机制由来自溶质载体家族的AA转运蛋白的一部分组成。这些转运蛋白具有双重功能;它们作为转运蛋白发挥作用，并参与血餐后脂肪体中激活的营养信号转导途径。在这项研究中，我们专注于溶质载体7家族（SLC 7），一个家庭的AA转运蛋白存在于所有后生动物，包括成员与强底物特异性的阳离子AA。我们确定了11个假定的SLC 7转运蛋白的基因组序列中的埃及伊蚊。系统发育分析将其中5个置于阳离子AA转运子亚家族（CAT）中，6个置于异二聚体AA转运子（HAT）亚家族中。所有11个埃及伊蚊SLC 7基因在成年雌性中表达。血餐后的表达谱是动态的。我们使用RNAi敲低了六种脂肪体表达的SLC 7转运蛋白，发现这些“敲低”减少了AA诱导的TOR信号传导。我们还确定了这些击倒对血餐后沉积的卵子数量的影响。我们的分析强调了SLC 7转运蛋白在TOR信号通路和蚊子繁殖中的重要性。
An important function of the fat body in adult female mosquitoes is the conversion of blood meal derived amino acids (AA) into massive amounts of yolk protein precursors. A highly efficient transport mechanism for AAs across the plasma membrane of the fat body trophocytes is essential in order to deliver building blocks for the rapid synthesis of large amounts of these proteins. This mechanism consists in part of AA transporter proteins from the solute carrier family. These transporters have dual function; they function as transporters and participate in the nutrient signal transduction pathway that is activated in the fat body after a blood meal. In this study we focused on the solute carrier 7 family (SLC7), a family of AA transporters present in all metazoans that includes members with strong substrate specificity for cationic AAs. We identified eleven putative SLC7 transporters in the genome sequence of Aedes aegypti. Phylogenetic analysis puts five of these in the cationic AA transporter subfamily (CAT) and six in the heterodimeric AA transporter (HAT) subfamily. All eleven Aedes aegypti SLC7 genes are expressed in adult females. Expression profiles are dynamic after a blood meal. We knocked down six fat body-expressed SLC7 transporters using RNAi and found that these ‘knockdowns’ reduced AA-induced TOR signaling. We also determined the effect these knockdowns had on the number of eggs deposited following a blood meal. Our analysis stresses the importance of SLC7 transporters in TOR signaling pathway and mosquito reproduction.