SARS-CoV-2 productively infects human gut enterocytes
SARS-CoV-2 productively infects human gut enterocytes
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DOI：
10.1126/science.abc1669
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发表时间：
2020-07-03
期刊：
影响因子：
56.9
通讯作者：
Clevers, Hans
中科院分区：
文献类型：
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作者：
Lamers, Mart M.;Beumer, Joep;Clevers, Hans
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）可引起2019冠状病毒病（COVID-19），这是一种流感样疾病，主要被认为通过呼吸道途径感染肺部。然而，临床证据表明肠道可能存在另一个病毒靶器官。事实上，SARS-CoV-2受体血管紧张素转换酶2（ACE 2）在分化的肠上皮细胞上高度表达。共聚焦显微镜和电子显微镜显示，在人类小肠类器官（hSlO）中，肠细胞容易被SARS-CoV和SARS-CoV-2感染。肠细胞产生感染性病毒颗粒，而hS 10的信使RNA表达分析揭示了通用病毒应答程序的诱导。因此，肠上皮支持SARS-CoV-2复制，并且hS 10充当冠状病毒感染和生物学的实验模型。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause coronavirus disease 2019 (COVID-19), an influenza-like disease that is primarily thought to infect the lungs with transmission through the respiratory route. However, clinical evidence suggests that the intestine may present another viral target organ. Indeed, the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) is highly expressed on differentiated enterocytes. In human small intestinal organoids (hSlOs), enterocytes were readily infected by SARS-CoV and SARS-CoV-2, as demonstrated by confocal and electron microscopy. Enterocytes produced infectious viral particles, whereas messenger RNA expression analysis of hSlOs revealed induction of a generic viral response program. Therefore, the intestinal epithelium supports SARS-CoV-2 replication, and hSlOs serve as an experimental model for coronavirus infection and biology.