Proteomic analysis provides insights into changes in the central metabolism of the cambium during dormancy release in poplar
Proteomic analysis provides insights into changes in the central metabolism of the cambium during dormancy release in poplar
复制标题
蛋白质组学分析为杨树休眠释放过程中形成层中央代谢的变化提供了见解
DOI：
10.1016/j.jplph.2016.10.007
复制
发表时间：
2017-01-01
影响因子：
4.3
通讯作者：
He, Xin-Qiang
中科院分区：
文献类型：
--
作者：
Jin, Feng;Li, Jing;He, Xin-Qiang
生长和休眠的季节性循环是温带木本植物的一个重要特征，休眠是一种应对冬季胁迫的有效策略。但是休眠维持及其解除的机制仍不清楚，特别是关于这一过程中蛋白质组的变化信息很少。更好地理解蛋白质的功能及其相关代谢途径将拓展我们对树木休眠维持及其解除机制的认识。在本研究中，我们采用同位素标记相对和绝对定量（iTRAQ）方法结合液相色谱 - 串联质谱（LC - MS/MS）分析来研究杨树休眠解除过程中的蛋白质谱变化。此外，通过组织化学方法研究了形成层中脂质、总不溶性碳水化合物和淀粉粒的变化。在杨树形成层组织中共鉴定出3789种蛋白质，其中1996种在休眠解除过程中发生了显著变化。通过功能分析，大多数发生变化的蛋白质涉及信号传导、植物激素、能量代谢、胁迫和次生代谢。我们的数据表明，在生态休眠和内休眠的解除阶段，脂质代谢蛋白质都发生了显著变化，而碳水化合物代谢蛋白质的变化主要在内休眠解除阶段。此外，组织化学结果与蛋白质组数据一致。我们的结果揭示了杨树在低温诱导的休眠解除过程中不同阶段特异性的代谢变化，这为树木休眠维持及其解除的调控机制提供了新的信息。（C）2016爱思唯尔集团。保留所有权利。
Seasonal cycling of growth and dormancy is an important feature for the woody plants growing in temperate zone, and dormancy is an effective strategy for surviving the winter stress. But the mechanisms of dormancy maintenance and its release are still not clear, especially little information is available with regard to the changes of proteome during the process. A better understanding in the function of proteins and their related metabolic pathways would expand our knowledge of the mechanisms of dormancy maintenance and its release in trees. In this study, we employed the isobaric tags for relative and absolute quantification (iTRAQ) approach with LC-MS/MS analysis to investigate the protein profile changes during dormancy release in poplar. In addition, the change of lipid, total insoluble carbohydrates and starch granules in the cambium was investigated by histochemical methods. A total of 3789 proteins were identified in poplar cambial tissues, 1996 of them were significantly altered during the dormancy release. Most of the altered proteins involved in signaling, phytohormone, energy metabolism, stress and secondary metabolism by functional analysis. Our data shows that the lipid metabolism proteins changed significantly both in the release stage of eco-and endodormancy, while the changes of carbohydrate metabolism proteins were mainly in endo-dormancy release stage. Moreover, histochemical results were consistent with the proteomic data. Our results reveal diverse stage-specific metabolism changes during the dormancy-release process induced by chilling in poplar, which provided new information regarding the regulation mechanisms of dormancy maintenance and its release in trees. (C) 2016 Elsevier GmbH. All rights reserved.