Growth hormone has dual stage-specific effects on the differentiation of 3T3-L1 preadipocytes.
Growth hormone has dual stage-specific effects on the differentiation of 3T3-L1 preadipocytes.
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生长激素对 3T3-L1 前脂肪细胞的分化具有双重阶段特异性作用。
DOI：
10.1093/oxfordjournals.jbchem.a003301
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发表时间：
2002
影响因子：
2.7
通讯作者：
T. Osumi
中科院分区：
文献类型：
--
作者：
S. Tominaga;M. Morikawa;T. Osumi
关于生长激素（GH）在脂肪细胞分化中的作用的报道各不相同。在这项研究中，我们使用无血清培养3T3-L1前脂肪细胞，发现生长激素根据分化阶段发挥双重作用。在激素鸡尾酒诱导分化过程中，在培养基中添加GH促进分化，但在处理后添加GH明显抑制分化。只有10%胎牛血清（FBS）存在时才有抑制作用。免疫消耗研究表明，生长激素有助于FBS的分化促进活性。胰岛素样生长因子-1不能复制生长激素的刺激或抑制作用。生长激素对分化的刺激涉及到中晚期脂肪细胞标志物mRNA表达的增强。在脂肪形成的关键调控因子中，过氧化物酶体增殖物激活受体(PPAR) γ和CCAAT/增强子结合蛋白(C/EBP) α在激素混合治疗期间被添加GH刺激mRNA表达，但C/EBP β未被刺激。生长激素对脂肪形成的刺激确实是由于分化细胞比例的增加，尽管生长激素也促进细胞生长。
Reports vary on the role of growth hormone (GH) in adipocyte differentiation. In this study, we showed that GH exerted dual effects depending on the stage of differentiation, using a serum-free culture of 3T3-L1 preadipocytes. GH promoted the differentiation when added to the medium during differentiation-inducing treatment with a hormone cocktail, but apparently suppressed it when added after the treatment. Only the suppressive effect was observed in the presence of 10% fetal bovine serum (FBS). Immunodepletion study showed that GH contributes to the differentiation-promoting activity of FBS. Insulin-like growth factor-1 could not replicate either the stimulative or the suppressive effect of GH. Stimulation of differentiation by GH involved the enhanced expression of mRNA of middle to late adipocyte markers. Among the key regulators of adipogenesis, peroxisome proliferator-activated receptor (PPAR) gamma and CCAAT/enhancer binding protein (C/EBP) alpha, but not C/EBPbeta, were stimulated for mRNA expression by GH added during the treatment with hormone cocktail. The stimulation of adipogenesis by GH was indeed due to the increase in the ratio of differentiated cells, though GH also promoted cell growth.