Analysis of cellular Mg2+ in DT40 cells.
Analysis of cellular Mg2+ in DT40 cells.
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DT40 细胞中细胞 Mg2 的分析。
DOI:
10.1007/978-1-4020-4896-8_28
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发表时间:
2006
影响因子:
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通讯作者:
Scharenberg,AndrewM
中科院分区:
文献类型:
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作者:
Perraud,Anne-Laure;Schmitz,Carsten;Scharenberg,AndrewM
Study of Mg2+ homeostasis in continuously growing cells requires the capacity to measure total cellular Mg2+ and net Mg2+ fluxes per unit time. Our laboratory's protocols for measurement of total cellular Mg2+ by atomic absorption spectrophotometry and measurement of net Mg2+ fluxes using the stable Mg2+ isotope 26Mg are described below.