CYTOKINE PRODUCTION IN A WHOLE-BLOOD ASSAY AFTER EPSTEIN-BARR-VIRUS INFECTION IN-VIVO
CYTOKINE PRODUCTION IN A WHOLE-BLOOD ASSAY AFTER EPSTEIN-BARR-VIRUS INFECTION IN-VIVO
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DOI：
10.1128/cdli.2.2.209-213.1995
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发表时间：
1995-03-01
期刊：
影响因子：
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通讯作者：
KIRCHNER, H
中科院分区：
文献类型：
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作者：
HORNEF, MW;WAGNER, HJ;KIRCHNER, H
EB病毒(Epstein-Barr Virus，EBV)对免疫系统的细胞有明显的趋向性，感染可导致深刻的免疫调节效应。为探讨细胞因子在传染性单核细胞增多症急性期的作用，我们检测了20例传染性单核细胞增多症患者血清中不同水平的白介素类(IL)、干扰素(IFN)和可溶性白介素2受体(sIL-2R)水平。我们发现IL-2、IL-6、sIL-2R和干扰素-γ水平升高。IL-2和IL-6浓度高峰出现在第一周(P<；0.01)，sIL-2R最大值出现在第二周(P<；0.01)。干扰素-γ水平仅在第一周内升高。此外，我们对11名患者和健康献血者进行了全血检测，观察了刺激有丝分裂刺激时产生细胞因子的能力。在内毒素刺激后患者的全血检测中，我们测量到肿瘤坏死因子α(P<；0.01)增加了10倍以上，IL-1β增加了3倍(P<；0.01)，IL-6增加了大约2倍(P<；0.01)。经植物血凝素刺激24 h后，sIL-2R和干扰素-γ浓度显著升高(P<；0.01)。总的来说，我们的数据似乎表明单核细胞在传染性单核细胞增多症期间被强烈激活。单核细胞和单核细胞衍生因子可能在传染性单核细胞增多症的发病机制中起重要作用，并与T淋巴细胞一起，可能是临床症状的部分原因。
Epstein-Barr virus (EBV) has a marked tropism for cells of the immune system, and infection can result in profound immunomodulatory effects. In order to examine the role of cytokines during the acute phase of infectious mononucleosis, we studied the levels of different interleukins (ILs), interferons (IFNs), and the soluble IL-2 receptor (sIL-2R) in serum samples of 20 patients. We found elevated levels of IL-2, IL-6, sIL-2R, and IFN-gamma. Whereas the peak of IL-2 and IL-6 concentration occurred during the first week (P < 0.01), the largest amounts of sIL-2R were measured during the second week (P < 0.01). IFN-gamma levels were only enhanced during the first week In addition, we investigated the ability to produce cytokines in response to mitogenic stimulation in a whole-blood assay of 11 patients compared with healthy blood donors. In the whole-blood assay of patients compared with controls after stimulation with lipopolysaccharide, we measured more than 10-fold elevated levels of tumor necrosis factor alpha (P < 0.01), 3-fold elevated levels of IL-1 beta (P < 0.01), and about 2-fold increased amounts of IL-6 (P < 0.01). A significant enhancement in sIL-2R and IFN-gamma concentration was found in the assay after stimulation with phytohemagglutinin after 24 h of incubation (P < 0.01). Collectively, our data seem to indicate that monocytes are strongly activated during infectious mononucleosis. Monocytes and monocyte-derived factors may play an important role in the pathogenesis of infectious mono-nucleosis and, together with T lymphocytes, may be partly responsible for clinical symptoms.