Differential gene expression patterns of the developing and adult mouse cornea compared to the lens and tendon
Differential gene expression patterns of the developing and adult mouse cornea compared to the lens and tendon
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DOI：
10.1016/j.exer.2008.06.001
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发表时间：
2008-09-01
影响因子：
3.4
通讯作者：
Chakravarti, Shukti
中科院分区：
文献类型：
--
作者：
Wu, Feng;Lee, Seakwoo;Chakravarti, Shukti
角膜在出生后继续成熟，形成功能齐全、屈光不正、具有保护性的屏障组织。在这里，我们通过描绘未成熟的出生后第10天和7周大的成年小鼠角膜的全球基因组范围的基因表达模式，研究了这一过程背后的复杂生物学事件。晶状体和肌腱被包括在研究中，以增加在角膜样本中被识别为上调的基因的特异性。角膜和肌腱之间基因表达的显著相似之处在于间充质细胞外基质胶原(I、III、V、VI型)和蛋白多糖(鲁米肯、核心蛋白聚糖和双聚糖)基因。在角膜和晶状体中的表达相似性仅限于某些上皮基因和晶体蛋白。大约76个基因在角膜样本中过度表达，这些基因在晶状体和肌腱中显示出基本的表达水平。其中32个是新的，在角膜中没有已知的功能。这些基因包括对氧化应激(Dhcr24，Cdo1，Akr1b7，Prdx6)、炎症(Ltb4dh，Wdr1)、离子转运(Pdzk1ip1，Slc12a2，Slc25a17)和转录(Zfp36l3，Pdzk1ip1)具有潜在作用的基因。对两个年龄段的角膜进行直接比较显示，P10和成人角膜中分别有50和12个基因选择性上调。在上调的P10基因中，有几个编码细胞外基质胶原蛋白和蛋白多糖，它们是成人角膜的稳定成分，它们在P10的高转录活性表明年轻角膜处于活跃的生长和基质沉积时期。关于这些基因在成人角膜中选择性地过度表达的了解要少得多；一些基因与免疫反应和神经支配(NPY)有关，可能与电子传递(CYP24A1、Cyp2f2)和其他尚不清楚的角膜功能有关(RGS10、Psmb8、Xlr4)。这项研究检测到具有已知功能的基因在角膜中的表达，为微阵列实验提供了额外的验证。重要的是，它发现了几个新的基因，它们的功能在角膜中还没有被研究过。(C)2008爱思唯尔有限公司。保留所有权利。
The cornea continues to mature after birth to develop a fully functional, refractive and protective barrier tissue. Here we investigated the complex biological events underlying this process by profiling global genome-wide gene expression patterns of the immature postnatal day 10 and 7-week old adult mouse cornea. The lens and tendon were included in the study to increase the specificity of genes identified as upregulated in the corneal samples. Notable similarities in gene expression between the cornea and the tendon were in the mesenchymal extracellular matrix collagen (types I, III, V, VI) and proteoglycan (lumican, decorin and biglycan) genes. Expression similarities in the cornea and lens were limited to certain epithelial genes and the crystallins. Approximately 76 genes were over expressed in the cornea samples that showed basal expression levels in the lens and tendon. Thirty-two of these were novel with no known functions in the cornea. These include genes with a potential role in protection against oxidative stress (Dhcr24, Cdo1, Akr1b7, Prdx6), inflammation (Ltb4dh, Wdr1), ion transport (Pdzk1ip1, Slc12a2, Slc25a17) and transcription (Zfp36l3, Pdzk1ip1). Direct comparison of the cornea of two ages showed selective upregulation of 50 and 12 genes in the P10 and adult cornea, respectively. Of the upregulated P10 genes several encode extracellular matrix collagens and proteoglycans that are stable components of the adult cornea and their high transcriptional activity at P10 indicate a period of actie corneal growth and matrix deposition in the young cornea. Much less is known about the genes selectively over expressed in the adult cornea; some relate to immune response and innervations (Npy), and possibly to electron transport (Cyp24a1, Cyp2f2) and others of yet unknown functions in the Cornea (Rgs10, Psmb8, Xlr4). This study detected expression of genes with known functions in the cornea, providing additional validation of the microarray experiments. Importantly, it identified several novel genes whose functions have not been investigated in the cornea. (C) 2008 Elsevier Ltd. All rights reserved.