Characterisation of the phosphorylcholine-containing N-linked oligosaccharides in the excretory-secretory 62 kDa glycoprotein of Acanthocheilonema viteae
Characterisation of the phosphorylcholine-containing N-linked oligosaccharides in the excretory-secretory 62 kDa glycoprotein of Acanthocheilonema viteae
复制标题
DOI：
10.1016/s0166-6851(96)02807-1
复制
发表时间：
1997-03-01
影响因子：
1.5
通讯作者：
Dell, A
中科院分区：
文献类型：
--
作者：
Haslam, SM;Khoo, KH;Dell, A
啮齿动物丝虫线虫Acanthocheilonema viteae的主要排泄分泌产物是62 kDa的糖蛋白（ES-62），其具有连接至N-连接的碳水化合物的磷酸胆碱。本文描述了由肽N-糖苷酶F从ES-62释放的N-聚糖的结构研究。观察到三种主要类型的N-聚糖结构：高甘露糖型结构;已完全修剪为三甘露糖基核心并进行亚化学计量岩藻糖基化的结构;以及具有三甘露糖基核心的结构，具有和不具有核心岩藻糖基化，携带1至4个额外的N-乙酰葡糖胺残基。在这三类聚糖中，只有最后一类被发现被可检测水平的磷酸胆碱取代。这些结果对A. viteae进行了讨论。(C)1997年Elsevier Science B.V.
The major excretory-secretory product of the rodent filarial nematode Acanthocheilonema viteae is a 62 kDa glycoprotein (ES-62), which has phosphorylcholine, attached to the N-linked carbohydrates. In this paper, we describe structural studies of N-glycans released from ES-62 by peptide N-glycosidase F. Three major classes of N-glycan structures were observed: high mannose type structures; those which had been fully trimmed to the trimannosyl core and were sub-stoichiometrically fucosylated; and those with a trimannosyl core, with and without core fucosylation, carrying between one and four additional N-acetylglucosamine residues. Of the three classes of glycans, only the last was found to be substituted with detectable levels of phosphorylcholine. The implications of these results with respect to the probable glycosylation pathways operating in A. viteae are discussed. (C) 1997 Elsevier Science B.V.