Expression of ABCA3 transporter gene in Tegillarca granosa and its association with cadmium accumulation
Expression of ABCA3 transporter gene in Tegillarca granosa and its association with cadmium accumulation
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粒蚴ABCA3转运蛋白基因的表达及其与镉积累的关系
DOI：
10.1016/j.gene.2022.146865
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发表时间：
2022-09-08
期刊：
影响因子：
3.5
通讯作者：
Teng，Shuangshuang
中科院分区：
文献类型：
--
作者：
Li，Zongfang;Li，Min;Teng，Shuangshuang
暴露于镉（Cd），一种重金属，可引起强烈的有毒副作用。Cd可通过多种途径进入生物体内，导致体内多种病理反应。泥蚶是我国沿海地区人们喜爱的一种双壳贝类。双壳贝类在滤食过程中容易吸收水体中的重金属污染物。泥蚶被认为是镉的超积累者，并且TgABCA 3基因在高含量镉暴露的泥蚶个体中高度表达。然而，TgABCA 3是否参与了镉离子在文蛤体内的转运，以及镉诱导文蛤细胞内环境稳定的分子机制尚不清楚。本研究对文蛤TgABCA 3基因的cDNA序列进行了分析，旨在为进一步研究TgABCA 3在文蛤抗镉过程中的作用提供理论依据。结果表明，TgABCA 3基因序列与其它双壳贝类TgABCA 3基因序列具有高度的同源性，并含有典型的ATP结合盒转运蛋白基序。组织特异性表达结果表明，TgABCA 3在鳃组织中表达量最高。使用RNA干扰技术沉默鳃组织中的TgABCA 3表达。TgABCA 3基因沉默后，TgABCA 3基因表达量下降，Cd含量增加，耗氧率和排氨率增加，摄食率下降。这些结果表明，镉积累的程度和由此产生的毒性效应与TgABCA 3的表达水平和活性有关，表明TgABCA 3在蛤体内对镉具有保护作用。镉累积量的增加会对人体造成严重损害，导致其生理代谢增强。因此，本研究结果表明TgABCA 3可以通过主动转运参与血蛤体内Cd离子的转运，在Cd解毒中发挥重要作用。
Exposure to cadmium (Cd), a heavy metal, can cause strong and toxic side effects. Cd can enter the body of organisms in several ways, leading to various pathological reactions in the body.Tegillarca granosais a kind of bivalve shellfish favored by people in the coastal areas of China. Bivalve shellfish can easily absorb heavy metal pollutants from water bodies while filter feeding.T. granosais considered a hyper-accumulator of Cd, and theTgABCA3gene is highly expressed in individuals with a high content of Cd-exposed blood clam. However, it is unclear whetherTgABCA3is involved in Cd ion transport in blood clam and the molecular mechanism for the mechanism of the Cd-induced responses for maintaining cell homeostasis. In this study, the complete cDNA of theTgABCA3gene was analyzed to provide insights into the roles ofTgABCA3in resistance against Cd in blood clam. The complete sequence ofTgABCA3showed high identity to that ofTgABCA3from other bivalves and contained some classical motifs of ATP-binding cassette transport proteins.TgABCA3expression in different tissues was measured using real-time quantitative polymerase chain reaction (qRT-PCR) and western blot analysis. The tissue-specific expression showed thatTgABCA3expression was highest in the gill tissue. TheTgABCA3expression in the gill tissue was silenced using the RNA interference technique. AfterTgABCA3silencing, theTgABCA3expression decreased, the Cd content increased, the oxygen consumption and ammonia excretion rates increased, and the ingestion rate decreased. These results showing that the extents of Cd accumulation and resulting toxic effects are related to expression levels and activity ofTgABCA3indicate that TgABCA3 has a protective function against Cd in the clam. This increase in Cd accumulation results in serious damage to the body, leading to the enhancement of its physiological metabolism. Therefore, the findings of the study demonstrated thatTgABCA3can participate in the transport of Cd ions in the blood clam through active transport and play a vital role in Cd detoxification.