Both RNase E and RNase III control the stability of sodB mRNA upon translational inhibition by the small regulatory RNA RyhB.
Both RNase E and RNase III control the stability of sodB mRNA upon translational inhibition by the small regulatory RNA RyhB.
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DOI：
10.1093/nar/gki313
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发表时间：
2005
影响因子：
14.9
通讯作者：
Kaberdin, VR
中科院分区：
文献类型：
--
作者：
Afonyushkin, T;Vecerek, B;Moll, I;Bläsi, U;Kaberdin, VR
先前的研究表明，sodB mRNA稳态浓度和可翻译性的铁依赖性变化是由小调控RNA RyhB、RNA伴侣Hfq和RNase E调节的。与RNase E的作用一致，我们发现在体内，当RNase E失活时，sodB mRNA的衰变被阻滞，并且酶在体外sodB 5 ' -非翻译区（5 ' -UTR）内切割。从而去除促进Hfq和核糖体结合的5 '茎环结构。此外，RNase E也可以发生在sodb5 ' -UTR/RyhB碱基配对时可用的隐位点上。我们发现Hfq虽然在促进RyhB与sodB mRNA的相互作用中发挥重要作用，但它并没有被RyhB - sodB mRNA复合物紧密保留，而是可以通过与反式中添加的其他rna相互作用而从中释放出来。与sodB mRNA的周转不同，RyhB在体内的衰变主要依赖于RNase III， RNase III在体外与sodB 5 ' -UTR碱基配对后促进其裂解。这些数据是根据一个模型来讨论的，该模型解释了RNase E和RNase III在sodB mRNA转换中所观察到的作用。
Previous work has demonstrated that iron-dependent variations in the steady-state concentration and translatability of sodB mRNA are modulated by the small regulatory RNA RyhB, the RNA chaperone Hfq and RNase E. In agreement with the proposed role of RNase E, we found that the decay of sodB mRNA is retarded upon inactivation of RNase E in vivo, and that the enzyme cleaves within the sodB 5′-untranslated region (5′-UTR) in vitro, thereby removing the 5′ stem–loop structure that facilitates Hfq and ribosome binding. Moreover, RNase E cleavage can also occur at a cryptic site that becomes available upon sodB 5′-UTR/RyhB base pairing. We show that while playing an important role in facilitating the interaction of RyhB with sodB mRNA, Hfq is not tightly retained by the RyhB–sodB mRNA complex and can be released from it through interaction with other RNAs added in trans. Unlike turnover of sodB mRNA, RyhB decay in vivo is mainly dependent on RNase III, and its cleavage by RNase III in vitro is facilitated upon base pairing with the sodB 5′-UTR. These data are discussed in terms of a model, which accounts for the observed roles of RNase E and RNase III in sodB mRNA turnover.
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影响因子：
5.6
作者：
Le Derout, J;Régnier, P;Hajnsdorf, E
通讯作者：
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影响因子：
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DOI：
10.1007/bf00330395
发表时间：
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期刊：
MOLECULAR AND GENERAL GENETICS
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