Improved specificity of hippocampal memory trace labeling.
Improved specificity of hippocampal memory trace labeling.
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DOI：
10.1002/hipo.22556
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发表时间：
2016-06
期刊：
影响因子：
3.5
通讯作者：
Denny CA
中科院分区：
文献类型：
--
作者：
Cazzulino AS;Martinez R;Tomm NK;Denny CA
最近的研究集中在啮齿动物模型的记忆痕迹的识别和操纵上。使用的两种主要小鼠模型是CreERT2/loxP他莫昔芬（TAM）或四环素反应因子(tTA)/四环素反应元件（TRE）多西环素（DOX）诱导系统。然而，这些系统可以改进，以标记与行为相对应的更具体的激活神经元群。在这里，我们试图鉴定一种改进的选择性雌激素受体（ER）调节剂（SERM），我们可以在ArcCreERT2小鼠中标记个体记忆痕迹。我们发现4-羟基他莫西芬（4-OHT）在ArcCreERT2 x ROSA26-CAG-stopflox-channelrhodospin （ChR2）增强的黄色荧光蛋白（eYFP）小鼠中是最具选择性的SERM。4-OHT的半衰期也比TAM短，可以更特异性地标记记忆痕迹。此外，4-OHT允许在齿状回（DG）和CA3中进行上下文特异性标记。总之，我们相信4-OHT提高了记忆痕迹标记的特异性，并将允许在未来进行精细的记忆痕迹研究。
Recent studies have focused on the identification and manipulation of memory traces in rodent models. The two main mouse models utilized are either a CreERT2/loxP tamoxifen (TAM)- or a tetracycline transactivator (tTA)/tetracycline-response element (TRE) doxycycline (DOX)-inducible system. These systems, however, could be improved to label a more specific population of activated neurons corresponding to behavior. Here, we sought to identify an improved selective estrogen receptor (ER) modulator (SERM) in which we could label an individual memory trace in ArcCreERT2 mice. We found that 4-hydroxytamoxifen (4-OHT) is the most selective SERM in the ArcCreERT2 x ROSA26-CAG-stopflox-channelrhodospin (ChR2)-enhanced yellow fluorescent protein (eYFP) mice. The half-life of 4-OHT is also shorter than TAM, allowing for more specificity of memory trace labeling. Furthermore, 4-OHT allowed for context-specific labeling in the dentate gyrus (DG) and CA3. In summary, we believe 4-OHT improves the specificity of memory trace labeling and will allow for refined memory trace studies in the future.