Platelet endothelial cell adhesion molecule, PECAM-1, modulates cell migration.
Platelet endothelial cell adhesion molecule, PECAM-1, modulates cell migration.
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血小板内皮细胞粘附分子 PECAM-1 调节细胞迁移。
DOI：
10.1002/jcp.1041530222
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发表时间：
1992
影响因子：
5.6
通讯作者：
Albelda,SM
中科院分区：
文献类型：
--
作者：
Schimmenti,LA;Yan,HC;Madri,JA;Albelda,SM
细胞迁移是生长、发育和伤口愈合等现象的重要过程。细胞迁移的控制部分是由细胞表面粘附分子协调的。这些分子分为两大类：与细胞外基质结合的分子和与邻近细胞结合的分子。在这里，我们报告了细胞间粘附分子血小板内皮细胞粘附分子-1（PECAM-1）（lg超家族的成员）在调节细胞迁移和细胞间粘附中的作用。 PECAM-1 是一种 120-130 kDa 的整合膜蛋白，存在于内皮细胞上并定位于细胞与细胞接触的部位。由于内皮细胞组成型表达 PECAM-1，我们研究了 PECAM-1 对空细胞群中细胞粘附和迁移的影响。具体来说，我们用全长 PECAM-1 分子（两个独立的克隆）转染 NIH/3T3 细胞。仅含有新霉素抗性基因的转染细胞、表达编码该分子胞外结构域的构建体的细胞和表达neu癌基因的细胞用作对照。 PECAM-1 转染子看起来更小、更呈多边形，并且倾向于成簇生长。 PECAM-1 转染子的间接免疫荧光显示细胞与细胞接触部位的外周染色，而胞外域转染子和对照细胞则没有。在两次定量迁移测定中，全长 PECAM-1 转染子的迁移速度比对照细胞慢。因此，转染到无效细胞中的 PECAM-1 似乎定位于细胞与细胞接触的位点，促进细胞与细胞的粘附，并降低迁移率。这些发现表明这种细胞-细胞粘附分子在内皮细胞迁移过程中发挥作用。 © 1992 Wiley-Liss, Inc.
Cell migration is an important process in such phenomena as growth, development, and wound healing. The control of cell migration is orchestrated in part by cell surface adhesion molecules. These molecules fall into two major categories: those that bind to extracellular matrix and those that bind to adjacent cells. Here, we report on the role of a cell‐cell adhesion molecule, platelet‐endothelial cell adhesion molecule‐1, (PECAM‐1), a member of the lg superfamily, in the modulation of cell migration and cell‐cell adhesion. PECAM‐1 is a 120–130 kDa integral membrane protein that resides on endothelial cells and localizes at sites of cell‐cell contact. Since endothelial cells express PECAM‐1 constitutively, we studied the effects of PECAM‐1 on cell‐cell adhesion and migration in a null‐cell population. Specifically, we transfected NIH/3T3 cells with the full length PECAM‐1 molecule (two independent clones). Transfected cells containing only the neomycin resistance gene, cells expressing a construct coding for the extracellular domain of the molecule, and cells expressing the neu oncogene were used as controls. The PECAM‐1 transfectants appeared smaller and more polygonal and tended to grow in clusters. Indirect immunofluorescence of PECAM‐1 transfectants showed peripheral staining at sites of cell‐cell contact, while the extracellular domain transfectants and the control cells did not. In two quantitative migration assays, the full‐length PECAM‐1 transfectants migrated more slowly than control cells. Thus, PECAM‐1 transfected into a null cell appears to localize to sites of cell‐cell contact, promote cell‐cell adhesion, and diminish the rate of migration. These findings suggest a role for this cell‐cell adhesion molecule in the process of endothelial cell migration. © 1992 Wiley‐Liss, Inc.
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血小板内皮细胞粘附分子-1 表达调节体外内皮细胞迁移。
DOI：
--
发表时间：
1998
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Kim,CS;Wang,T;Madri,JA
通讯作者：
Madri,JA
DOI：
10.1073/pnas.94.12.6273
发表时间：
1997-06-10
影响因子：
11.1
作者：
Vittet, D;Buchou, T;Huber, P
通讯作者：
Huber, P
影响因子：
6.5
作者：
BRINKMAN, HJM;MERTENS, K;VANMOURIK, JA
通讯作者：
VANMOURIK, JA
DOI：
10.1152/ajpheart.1997.273.2.h981
发表时间：
1997-08-01
影响因子：
4.8
作者：
Hixenbaugh, EA;Goeckeler, ZM;Huang, AJ
通讯作者：
Huang, AJ
影响因子：
7.8
作者：
DelMaschio, A;Zanetti, A;Corada, M;Rival, Y;Ruco, L;Lampugnani, MG;Dejana, E
通讯作者：
Dejana, E