INCREASING LEVELS OF MYC AND MET COAMPLIFICATION DURING TUMOR PROGRESSION OF A CASE OF GASTRIC-CANCER
INCREASING LEVELS OF MYC AND MET COAMPLIFICATION DURING TUMOR PROGRESSION OF A CASE OF GASTRIC-CANCER
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DOI：
10.1016/0165-4608(95)00033-l
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发表时间：
1995-07-15
影响因子：
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通讯作者：
SOBRINHOSIMOES, M
中科院分区：
文献类型：
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作者：
SERUCA, R;SUIJKERBUIJK, RF;SOBRINHOSIMOES, M
对胃癌结节转移的细胞遗传学研究表明，在裸鼠体内传代两代后，出现了大量的双分钟。用提取的DNA进行比较基因组原位杂交(CGH)分析表明，异种移植DNA中存在3个清晰的扩增单位，分别来自染色体6q24-25、7q31-32和8q24亚区。从原发肿瘤及其转移瘤提取的DNA中也发现了类似的CGH结果，尽管不那么显著，这意味着在这些肿瘤组织的DNA中也存在相同的扩增片段，尽管丰度较低。对第二代异种移植瘤的Southern分析检测到MET(位于7q31)和MYC(位于8q24)分别扩增了18倍和10倍。对第一代异种移植瘤以及移植前转移和原发肿瘤的回顾性研究显示，MET扩增水平分别为12倍、9倍和5倍，MYC扩增水平分别为8倍、7倍和5倍。我们的结果表明，MYC和MET共扩增水平的增加与胃癌生长潜力的增强有关。
The cytogenetic study of a nodal metastasis From a gastric carcinoma, after two passages in nude mice, revealed a large number of double minutes. Comparative genomic in situ hybridization (CGH) analysis using DNA extracted from this xenograft revealed the existence of three clear amplification units that originated from the chromosomal subregions 6q24-25, 7q31-32, and 8q24 in the xenograft DNA. Similar, though less prominent, CGH results were found with DNAs extracted from the primary tumor and its metastasis, implying that the same amplicons were also present, albeit less abundantly, in the DNAs of these neoplastic tissues. Southern analysis of the second-passage xenograft detected 18- and 10-fold amplification of MET (located at 7q31) and MYC (located at 8q24), respectively. The retrospective study of the first passage of the xenograft, as well as of the metastatic and primary tumors before xenografting, showed amplification levels of MET of, respectively 12; 9-, and 5-fold and MYC of, respectively, 8, 7-, and 5-fold. Our results suggest that increased levels of co-amplification of MYC and MET correlate with enhanced growth potential in this case of gastric carcinoma.