Modeling and immunohistochemical analysis of C6 glioma In Vivo
Modeling and immunohistochemical analysis of C6 glioma In Vivo
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DOI：
10.1007/s10517-007-0167-y
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发表时间：
2007-04-01
影响因子：
0.7
通讯作者：
Gurina, O. I.
中科院分区：
文献类型：
--
作者：
Chekhonin, V. P.;Baklaushev, V. P.;Gurina, O. I.
建立了Wistar大鼠C6胶质瘤的体内可复制模型。组织学分析显示大鼠C6胶质瘤与人胶质母细胞瘤形态相似。免疫组织化学方法显示胶质瘤周围形成胶质边界，由gmap阳性反应星形胶质细胞组成。植入后第8天出现边界，观察星形胶质细胞形成，直至动物死亡（第28天）。具有分支突的反应性星形胶质细胞不仅包围了原发性胶质瘤病灶，而且包围了神经组织中肿瘤侵袭的所有部位。在肿瘤内皮细胞中，EBA（血脑屏障标志物）的表达受到干扰，AMVB1（内皮抗原）的合成增加，这表明与血脑屏障相比，血肿瘤屏障的功能重构明显。GFAP和AMVB1在肿瘤组织中的优势表达现象可用于通过适当的抗体开发靶向药物转运到肿瘤中的系统。
A reproducible in vivo model of C6 glioma was developed in Wistar rats. Analysis of histological preparations showed similar morphology of rat C6 glioma and human glioblastoma. The formation of a glial border at the periphery of the glioma, consisting of GFAP-positive reactive astrocytes, was shown by the immunohistochemical method. The border appeared on day 8 after implantation, astrogliosis was observed until animal death (day 28). Reactive astrocytes with branched processes surrounded not only the primary glioma focus, but also all sites of tumor invasion in the nervous tissue. Expression of EBA (blood-brain barrier marker) was disturbed and synthesis of AMVB1 (endothelial antigen) increased in neoplastic endotheliocytes, which suggested pronounced functional restructuring of the blood-tumor barrier in comparison with the blood-brain barrier. The phenomenon of predominant expression of GFAP and AMVB1 in the tumor tissue can be used for the development of systems for targeted drug transport into the tumor by means of appropriate antibodies.