Genetic recombination in brome mosaic virus: effect of sequence and replication of RNA on accumulation of recombinants.
Genetic recombination in brome mosaic virus: effect of sequence and replication of RNA on accumulation of recombinants.
复制标题
雀麦花叶病毒的基因重组：RNA 序列和复制对重组体积累的影响。
DOI：
10.1128/jvi.66.11.6824-6828.1992
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发表时间：
1992
影响因子：
5.4
通讯作者：
Bujarski,JJ
中科院分区：
文献类型：
--
作者：
Nagy,PD;Bujarski,JJ
为了便于分离雀麦花叶病毒的重组体，设计了一系列在RNA 3 3'非编码区具有改变的重复突变体。交叉的分布，这被观察到是依赖于亲本RNA3序列，支持重组中的RNA结构的作用。然而，亲本RNA3构建体的复制和重组后代的积累之间的负相关性证实了选择的作用。
In order to facilitate the isolation of recombinants in brome mosaic virus, a series of duplication mutants with alterations in the RNA3 3' noncoding region has been engineered. The distribution of crossovers, which was observed to be dependent on the parental RNA3 sequence, supported the role of RNA structure in recombination. However, a negative correlation between replication of the parental RNA3 constructs and the accumulation of recombinant progeny confirmed the role of selection.