Metabolism of n-3 polyunsaturated fatty acids and modification of phospholipids in cultured rabbit aortic smooth muscle cells.
Metabolism of n-3 polyunsaturated fatty acids and modification of phospholipids in cultured rabbit aortic smooth muscle cells.
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培养兔主动脉平滑肌细胞中n-3多不饱和脂肪酸的代谢和磷脂的修饰。
DOI：
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发表时间：
1985
影响因子：
6.5
通讯作者：
S. Yoshida
中科院分区：
文献类型：
--
作者：
N. Morisaki;T. Kanzaki;Y. Fujiyama;I. Osawa;K. Shirai;N. Matsuoka;Y. Saito;S. Yoshida
将培养的兔主动脉平滑肌细胞中脂肪酸的亚麻酸家族 (n-3)（例如亚麻酸、二十碳五烯酸和二十二碳六烯酸）的代谢与亚油酸和花生四烯酸的代谢进行比较。细胞对这些脂肪酸的吸收呈时间依赖性，持续 16 小时（花生四烯酸大于二十二碳六烯酸，亚油酸，二十碳五烯酸大于亚麻酸），并且这些酸主要掺入磷脂和甘油三酯中。与亚麻酸和亚油酸相比，二十碳五烯酸和花生四烯酸更多地掺入磷脂酰乙醇胺和磷脂酰肌醇加磷脂酰丝氨酸中，而更少地掺入磷脂酰胆碱中。二十二碳六烯酸比亚麻酸和亚油酸更多地掺入磷脂酰乙醇胺中，并且比二十碳五烯酸和花生四烯酸更少地掺入磷脂酰肌醇和磷脂酰丝氨酸中。添加的亚麻酸主要在磷脂酰胆碱中积累，并且不会降低任何磷脂亚组分的花生四烯酸含量。亚油酸的伸长去饱和代谢物没有积累。用二十碳五烯酸处理的细胞主要在磷脂酰乙醇胺中积累二十碳五烯酸和二十二碳五烯酸，并且花生四烯酸含量降低。添加的二十二碳六烯酸主要在磷脂酰乙醇胺中积累，并降低了花生四烯酸和油酸的含量。从这些结果得出以下结论。三种 n-3 脂肪酸在磷脂中的利用方式不同。磷脂的花生四烯酸含量会被二十碳五烯酸和二十二碳六烯酸降低，但不会被亚麻酸降低。平滑肌细胞几乎没有或没有去饱和酶活性，但对多不饱和脂肪酸具有显着的延伸活性。
The metabolism of the linolenic acid family (n-3) of fatty acids, e.g., linolenic, eicosapentaenoic, and docosahexaenoic acids, in cultured smooth muscle cells from rabbit aorta was compared to the metabolism of linoleic and arachidonic acids. There was a time-dependent uptake of these fatty acids into cells for 16 hr (arachidonic greater than docosahexaenoic, linoleic, eicosapentaenoic greater than linolenic), and the acids were incorporated mainly into phospholipids and triglycerides. Eicosapentaenoic and arachidonic acids were incorporated more into phosphatidylethanolamine and phosphatidylinositol plus phosphatidylserine and less into phosphatidylcholine than linolenic and linoleic acids. Docosahexaenoic acid was incorporated into phosphatidylethanolamine more than linolenic and linoleic acids and into phosphatidylinositol plus phosphatidylserine less than eicosapentaenoic and arachidonic acids. Added linolenic acid accumulated mainly in phosphatidylcholine and did not decrease the arachidonic acid content of any phospholipid subfraction. Elongation-desaturation metabolites of linoleic acid did not accumulate. Cells treated with eicosapentaenoic acid accumulated both eicosapentaenoic and docosapentaenoic acids mainly in phosphatidylethanolamine and the arachidonic acid content was decreased. Added docosahexaenoic acid accumulated mainly in phosphatidylethanolamine and decreased the content of both arachidonic and oleic acids. The following conclusions are drawn from these results. The three n-3 fatty acids are utilized differently in phospholipids. The arachidonic acid content of phospholipids is reduced by eicosapentaenoic and docosahexaenoic acids, but not by linolenic acid. Smooth muscle cells have little or no desaturase activity, but have significant elongation activity for polyunsaturated fatty acids.
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影响因子：
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作者：
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影响因子：
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DOI：
10.1016/0006-291x(83)91235-4
发表时间：
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影响因子：
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作者：
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通讯作者：
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影响因子：
13.6
作者：
Spector,AA;Hoak,JC;Fry,GL;Stoll,LL;Tanke,CT;Kaduce,TL
通讯作者：
Kaduce,TL