Nuclear magnetic resonance characteristics of fresh and fixed tissue: the effect of elapsed time.
Nuclear magnetic resonance characteristics of fresh and fixed tissue: the effect of elapsed time.
复制标题
新鲜和固定组织的核磁共振特征：经过时间的影响。
作者：
D. Thickman;H. Kundel;G. Wolf
用生理盐水、70%乙醇固定、中性缓冲福尔马林、10%福尔马林固定和未处理的大鼠肝、脾标本，在48小时内测定肝和脾的核磁共振弛豫时间T1和T2。两种组织的t1和t2的初始值与先前的测定结果一致。未经处理的肝、脾组织，T1稳定6h，T2稳定48h。对于固定的组织，T1在0.5h内稳定，而T2在0.5h内发生变化。
The nuclear magnetic resonance relaxation times T1 and T2 of liver and spleen from freshly euthanized rats were measured over 48 hours for samples treated with normal saline, fixed with 70% ethanol, neutral buffered formalin, or 10% formalin, or left untreated. The initial values of T1 and T2 for both tissues agreed with previous determinations. For untreated tissues of both liver and spleen, T1 was stable for 6 hours and T2 was stable for 48 hours. For fixed tissues, T1 was stable for 0.5 hours, but T2 changed in 0.5 hours.