Independent amplification and frequent co-amplification of three nonsyntenic regions on the long arm of chromosome 20 in human breast cancer.
Independent amplification and frequent co-amplification of three nonsyntenic regions on the long arm of chromosome 20 in human breast cancer.
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发表时间：
1996-08
期刊：
影响因子：
11.2
通讯作者：
M. Tanner;M. Tirkkonen;A. Kallioniemi;J. Isola;T. Kuukasjärvi;Colin Collins;Colin Collins;David Kowb
中科院分区：
文献类型：
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作者：
M. Tanner;M. Tirkkonen;A. Kallioniemi;J. Isola;T. Kuukasjärvi;Colin Collins;Colin Collins;David Kowb
20q13.2的DNA扩增在乳腺癌中很常见，与预后不良相关，可能反映了一个重要癌基因的位置。最近，沿着20q的其他区域也被发现出现了扩增。本文采用间期荧光原位杂交技术，分析了146例未培养乳腺癌和14株细胞系中沿20q的14个位点的共扩增水平和模式。在未培养的肿瘤中有三个区域独立扩增：20q13.2的RMC20C001区域（9.6%的病例高度扩增），PTPN1区域3mb近端（6.2%）和20q11的AIB3区域（6.2%）。19例高度扩增的肿瘤中有11例共扩增涉及两个或三个区域。结果表明，沿20q的三个不同的非同型区域可能是重要的，复杂的染色体重排是乳腺癌中它们频繁共扩增的基础。
DNA amplification at 20q13.2 is common in breast cancer, correlates with poor prognosis, and may reflect location of an important oncogene. Recently, other regions along 20q were also found to undergo amplification. Here, amplification levels and patterns of co-amplification were analyzed by interphase fluorescence in situ hybridization at 14 loci along 20q in 146 uncultured breast carcinomas and 14 cell lines. Three regions were independently amplified in uncultured tumors: RMC20C001 region at 20q13.2 (highly amplified in 9.6% of the cases), PTPN1 region 3 Mb proximal (6.2%), and AIB3 region at 20q11 (6.2%). Co-amplifications involving two or three of these regions were seen in 11 of the 19 highly amplified tumors. The results suggest that three distinct nonsyntenic regions along 20q may be important and that complex chromosomal rearrangements underlie their frequent co-amplification in breast cancer.