Comparison of global gene expression between porcine testis tissue xenografts and porcine testis in situ.
Comparison of global gene expression between porcine testis tissue xenografts and porcine testis in situ.
复制标题
猪睾丸组织异种移植物与猪睾丸原位基因表达的比较。
DOI：
10.1002/mrd.20670
复制
发表时间：
2007
影响因子：
2.5
通讯作者：
Dobrinski,Ina
中科院分区：
文献类型：
--
作者：
Zeng,Wenxian;Rathi,Rahul;Pan,Hua;Dobrinski,Ina
Testis tissue from immature mammalian donor animals, grafted ectopically to immunodeficient mouse hosts, can undergo complete spermatogenesis with the production of fertilization‐competent spermatozoa. To further characterize testis tissue xenografts as a model for testis function in situ, the objective of this study was to compare gene expression between porcine testis tissue xenografts and testis tissue in situ. Pieces of testis tissue from 1‐week‐old piglets were grafted onto immunodeficient male mice and a littermate piglet was raised for comparison as control. Complete spermatogenesis was present in the testis tissue xenografts at 8 months after transplantation into mouse hosts and in the 8‐month‐old control porcine testis tissue. Total RNA was isolated from xenografts and control tissue, and the RNA was labeled and hybridized to the porcine genome array. By analyzing the expression of 23,256 transcripts, we found that 71 genes were differentially expressed with at least a fourfold difference between xenografts and control tissue. Interestingly, none of the 56 transcripts present on the array that were annotated in porcine testis showed differential expression between xenografts and control testis. This analysis indicates that global gene expression in porcine testis xenografts appears comparable to testis tissue in situ. These findings support the hypothesis that testis tissue xenografts can provide a representative model to study mammalian spermatogenesis. Mol. Reprod. Dev. 74: 674–679, 2007. © 2006 Wiley‐Liss, Inc.
