IMPROVED SANDWICH ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR TRANSFORMING GROWTH FACTOR-BETA-1
IMPROVED SANDWICH ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR TRANSFORMING GROWTH FACTOR-BETA-1
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DOI：
10.1016/0022-1759(93)90254-5
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发表时间：
1993-01-14
影响因子：
2.2
通讯作者：
DANIELPOUR, D
中科院分区：
文献类型：
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作者：
DANIELPOUR, D
转化生长因子β是一个由25 kDa二聚体组成的高度同源的家族，具有旁分泌和自分泌的生长调节作用，传递着广泛的重要生物学功能。尽管已经描述了对转化生长因子-β的敏感生物测定，但它们被其他生长调节剂的干扰限制了它们的应用。以兔抗转化生长因子-β1为捕获抗体，建立了一种高灵敏度(20-50pg/ml)、高特异性的夹心酶联免疫吸附试验检测转化生长因子-β1的方法。这种相当低效价抗体的可获得性限制了这种检测方法的广泛使用。这份报告描述了改良的转化生长因子-β1-SELISA，对上述检测具有类似的敏感性，基于可获得的商业单抗和多克隆抗体来源。这些新开发的SELISA是测定复杂生物体液中转化生长因子-β1的最敏感、最特异、最准确和最方便的方法。
Transforming growth factor betas (TGF-betas), a highly homologous family of 25 kDa dimers, function as both paracrine and autocrine growth modulators which relay a broad spectrum of critical biological functions. Although sensitive bioassays for TGF-betas have been described, their interference by other growth modulators have limited their application. We have previously described a highly sensitive (20-50 pg/ml) and specific sandwich enzyme-linked immunosorbent assay (SELISA) for TGF-beta1, based on use of rabbit anti-TGF-beta1, as the capture antibody. Widespread use of this assay was limited by the availability of this rather low-titer antibody. This report describes improved TGF-beta1 SELISAs with comparable sensitivities to the above assay based on available sources of commercial monoclonal and polyclonal antibodies. These newly developed SELISAs are the most sensitive, specific, precise and convenient methods for the quantitation of TGF-beta1 in complex biological fluids.