Rapid determination of lipid peroxidation using a novel pyridoxamine-participating ferrous oxidation-sulfosalicylic acid spectrophotometric method
Rapid determination of lipid peroxidation using a novel pyridoxamine-participating ferrous oxidation-sulfosalicylic acid spectrophotometric method
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DOI：
10.1016/j.foodchem.2016.05.106
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发表时间：
2016-11-15
期刊：
影响因子：
8.8
通讯作者：
Zhang, Yu
中科院分区：
文献类型：
--
作者：
Chen, Jingnan;Cai, Danqian;Zhang, Yu
建立了一种新的方法，即吡哆胺参与亚铁氧化-磺基水杨酸（PFOS）法，可在室温下快速测定食用油和油脂类食品中的脂质过氧化。全氟辛烷磺酸方法通过在500 nm处检测吡哆胺介导的由5-磺基水杨酸和Fe 3+产生的色素（而Fe 3+是在脂质过氧化物存在的情况下由Fe 2+转化而来的），以比色法评估脂质过氧化物值。最佳处方为乙醇（体积分数70%）、Fe 2+（4 mmol/L）、5-磺基水杨酸（40 mmol/L）和吡哆胺（18 mmol/L）。定量限为0.087 mmol Fe 3 +/L，重现性可接受。此外，本方法与传统的硫代巴比妥酸（R-2 = 0.9999）和硫氰酸铁（R-2 = 0.9675）检测方法具有显著的线性相关性。该方法提供了一种快速评价脂质过氧化反应的技术，无需加热和复杂的仪器程序。此外，本方法为评价脂质过氧化状态和提高亚铁氧化的重现性提供了一种新的选择。(C)2016爱思唯尔有限公司版权所有
A novel method is developed to rapidly analyze lipid peroxidation in edible oils and fatty foods at room temperature, which is called the pyridoxamine-participating ferrous oxidation-sulfosalicylic acid (PFOS) method. The PFOS method evaluates the lipid peroxide value colorimetrically via detecting the pyridoxamine-mediated pigment produced by 5-sulfosalicylic acid and Fe3+ at 500 nm, while the latter is converted from Fe2+ in the presence of lipid peroxides. The optimized formulation was ethanol (70%, v/v), Fe2+ (4 mmol/L), 5-sulfosalicylic acid (40 mmol/L) and pyridoxamine (18 mmol/L). The limit of quantitation is 0.087 mmol Fe3+/L with acceptable reproducibility. In addition, current method has a significant linear correlation with both conventional thiobarbituric acid (R-2 = 0.9999) and ferric thiocyanate assays (R-2 = 0.9675). This method offers a rapid technique for evaluating lipid peroxidation without heating and sophisticated instrumental procedures. Besides, current method provides a new option to evaluate the lipid peroxidation state and improve the reproducibility of ferrous-oxidation. (C) 2016 Elsevier Ltd. All rights reserved.