Comparative Proteomics Indicates That Redox Homeostasis Is Involved in High- and Low-Temperature Stress Tolerance in a Novel Wucai (Brassica campestris L.) Genotype
Comparative Proteomics Indicates That Redox Homeostasis Is Involved in High- and Low-Temperature Stress Tolerance in a Novel Wucai (Brassica campestris L.) Genotype
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比较蛋白质组学表明氧化还原稳态与新五彩基因型的高温和低温应激耐受性有关
DOI：
10.3390/ijms20153760
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发表时间：
2019-08-01
影响因子：
5.6
通讯作者：
Wang, Chenggang
中科院分区：
文献类型：
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作者：
Yuan, Lingyun;Wang, Jie;Wang, Chenggang
WS-1基因型是以前从新的五彩种质中鉴定的，既耐低温（LT）又耐高温（HT）。然而，目前还不清楚哪些信号转导途径或驯化机制参与了温度胁迫反应。在这项研究中，我们使用串联质量标签（TMT）与液相色谱-质谱（LC-MS/MS）相结合的蛋白质组学方法，以确定1022差异表达蛋白（DEPs）共同WS-1，无论是LT或HT处理。在这1022个DEP中，172个响应于LT和HT而上调，324个响应于LT和HT而下调，526个响应于一种温度胁迫而上调，响应于另一种温度胁迫而下调。为了说明WS-1中的共同调控途径，进一步分析了172种上调的DEP。氧化还原平衡、光合作用、碳水化合物代谢、热激蛋白、分子伴侣和信号转导途径与五彩的温度胁迫耐性有关。此外，在拟南芥中过表达的35 S：BcccrGLU 1在温度胁迫下表现出较高的还原型谷胱甘肽（GSH）含量和还原型谷胱甘肽/氧化型谷胱甘肽（GSH/GSSG）比值，且氧化损伤较小。这一结果与参与氧化还原稳态的相关蛋白质的动态调节一致。这些数据表明，维持氧化还原稳态是一个重要的共同调节途径，在新的五彩种质耐温度胁迫。
The genotype WS-1, previously identified from novel wucai germplasm, is tolerant to both low-temperature (LT) and high-temperature (HT) stress. However, it is unclear which signal transduction pathway or acclimation mechanisms are involved in the temperature-stress response. In this study, we used the proteomic method of tandem mass tag (TMT) coupled with liquid chromatography-mass spectrometry (LC-MS/MS) to identify 1022 differentially expressed proteins (DEPs) common to WS-1, treated with either LT or HT. Among these 1022 DEPs, 172 were upregulated in response to both LT and HT, 324 were downregulated in response to both LT and HT, and 526 were upregulated in response to one temperature stress and downregulated in response to the other. To illustrate the common regulatory pathway in WS-1, 172 upregulated DEPs were further analyzed. The redox homeostasis, photosynthesis, carbohydrate metabolism, heat-shockprotein, and chaperones and signal transduction pathways were identified to be associated with temperature stress tolerance in wucai. In addition, 35S:BcccrGLU1 overexpressed in Arabidopsis, exhibited higher reduced glutathione (GSH) content and reduced glutathione/oxidized glutathione (GSH/GSSG) ratio and less oxidative damage under temperature stress. This result is consistent with the dynamic regulation of the relevant proteins involved in redox homeostasis. These data demonstrate that maintaining redox homeostasis is an important common regulatory pathway for tolerance to temperature stress in novel wucai germplasm.