Differential CARS microscopy with linearly chirped femtosecond laser pulses
Differential CARS microscopy with linearly chirped femtosecond laser pulses
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DOI：
10.1117/12.873872
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发表时间：
2011-02
期刊：
影响因子：
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通讯作者：
W. Langbein;I. Rocha-Mendoza;F. Masia;C. Di Napoli;I. Pope;P. Watson;P. Borri
中科院分区：
文献类型：
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作者：
W. Langbein;I. Rocha-Mendoza;F. Masia;C. Di Napoli;I. Pope;P. Watson;P. Borri
我们利用高群速色散玻璃元件线性啁啾的飞秒激光脉冲，实现了频率差分CARS(D-CARS)。通过将泵浦-斯托克斯对以两倍的激光重复频率复制成脉冲序列，并通过玻璃色散控制瞬时频差，我们以一种本质上稳定的方式调节了每对探测到的拉曼频率。由此产生的CARS强度由单个光电倍增管使用锁定检测同时检测为和和差。我们展示了具有强烈抑制的非共振背景的活细胞的成像。我们还展示了使用单个飞秒激光光源的D-CARS。
We demonstrate frequency differential CARS (D-CARS) using femtosecond laser pulses linearly chirped by glass elements of high group-velocity dispersion. By replicating the Pump-Stokes pair into a pulse train at twice the laser repetition rate, and controlling the instantaneous frequency difference by glass dispersion, we adjust the Raman frequency probed by each pair in an intrinsically stable way. The resulting CARS intensities are detected simultaneously by a single photomultiplier as sum and difference using lock-in detection. We demonstrate imaging of living cells with strongly suppressed non-resonant background. We also show D-CARS using a single femtosecond laser source.