Functional Changes in the Retina during and after Acute Intraocular Pressure Elevation in Mice
Functional Changes in the Retina during and after Acute Intraocular Pressure Elevation in Mice
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DOI：
10.1167/iovs.09-3814
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发表时间：
2009-12-01
影响因子：
4.4
通讯作者：
Bui, Bang V.
中科院分区：
文献类型：
--
作者：
Kong, Yu X.;Crowston, Jonathan G.;Bui, Bang V.
目的.采用全视野视网膜电图（ERG）检测野生型小鼠急性眼内压（IOP）升高时及升高后的视网膜功能。在野生型C57/BL 6小鼠中，前房插管导致IOP升高。压力-函数关系通过从基线到80 mm Hg逐步升高的IOP来确定。在50 mm Hg的IOP峰值持续30分钟后，评估60分钟的功能恢复率。在IOP升高期间和之后立即记录暗视ERG信号对暗淡和明亮闪光的反应（-4.54、-2.23和0.34 log cd.s.m（-2）），并分析光感受性（a波）、ON双极（b波）、振荡电位（OPs）和暗视阈值反应（阳性[p] STR/阴性[n] STR）。在单次50 mm Hg IOP峰值之前2天和之后7天收集完整的ERG方案。pSTR对IOP升高最敏感，在41 mm Hg下50%振幅损失（mu）（mu，95%置信限（CL）：37.7，45.6），其次是45 mm Hg下nSTR（95% CL：41.0，49.1）。pSTR的敏感性显著高于b波（95% CL：41.4，49.1）、a波（95% CL：47.6，55.3）和OP（95% CL：49.6，59.2）。与b波相比，pSTR在50 mm Hg尖峰后立即显示出较慢的恢复（P = 0.02）。在50 mm Hg峰值后一周，pSTR（-30% +/-6%，P < 0.001）和OP（-27% +/-2%，P < 0.001）振幅降低，而其他成分未受影响。小鼠的STR对急性IOP升高更敏感，并且比其他ERG成分恢复更慢。1周时pSTR和OP振幅的降低表明在单次IOP尖峰后可能发生视网膜内功能的持续损害。（Invest Ophthalmol维斯科学。2009; 50：5732-5740）DOI：10.1167/iovs.09-3814
PURPOSE. To examine retinal function using the full-field electroretinogram (ERG) during and after acute intraocular pressure (IOP) elevation in wild-type mice.METHODS. IOP was elevated by anterior chamber cannulation in wild-type C57/BL6 mice. The pressure-function relationship was determined by IOP elevation in steps from baseline to 80 mm Hg. The rate of functional recovery was assessed for 60 minutes after an IOP spike of 50 mm Hg for 30 minutes. During and immediately after IOP elevation, scotopic ERG signals were recorded in response to dim and bright flashes (-4.54, -2.23, and 0.34 log cd.s.m(-2))and analyzed for photoreceptoral (a-wave), ON-bipolar (b-wave), oscillatory potentials (OPs), and scotopic threshold responses (positive [p] STR/negative [n] STR). A full ERG protocol was collected 2 days before and 7 days after the single 50-mm Hg IOP spike.RESULTS. The pSTR was most sensitive to IOP elevation with 50% amplitude loss (mu) at 41 mm Hg (mu, 95% confidence limits (CL): 37.7, 45.6) followed by nSTR at 45 mm Hg (95% CL: 41.0, 49.1). pSTR was significantly more sensitive than the b-wave (95% CL: 41.4, 49.1), a-wave (95% CL: 47.6, 55.3), and OPs (95% CL: 49.6, 59.2). pSTR showed slower recovery immediately after the 50 mm Hg spike compared with the b-wave (P = 0.02). One week after the 50-mm Hg spike, pSTR (-30% +/- 6%, P < 0.001) and OP (-27% +/- 2%, P < 0.001) amplitudes were reduced, whereas other components were unaffected.CONCLUSIONS. The STR in mice is more sensitive to acute IOP elevation and recovers slower than other ERG components. Reduction in pSTR and OP amplitude at 1 week suggests persistent impairment of inner retinal function can occur after a single IOP spike. (Invest Ophthalmol Vis Sci. 2009; 50: 5732-5740) DOI:10.1167/iovs.09-3814