MEASUREMENT OF INCORPORATION OF RADIOACTIVE AMINO ACIDS INTO PROTEIN BY A FILTER-PAPER DISK METHOD
MEASUREMENT OF INCORPORATION OF RADIOACTIVE AMINO ACIDS INTO PROTEIN BY A FILTER-PAPER DISK METHOD
复制标题
DOI：
10.1016/0003-9861(61)90009-1
复制
发表时间：
1961-01-01
影响因子：
3.9
通讯作者：
NOVELLI, GD
中科院分区：
文献类型：
--
作者：
MANS, RJ;NOVELLI, GD
样品（0.02 ~ 0.12 ml）从C14或H3标记的氨基酸合并系统中取出，应用于2.3 cm滤纸盘，酸沉淀，洗涤和批量提取，最后在液体闪烁光谱仪中直接计数。检测到的计数是添加到磁盘上的放射性量的线性函数；在放射性变化50倍和蛋白质浓度变化15倍的情况下，它与比活性、蛋白质总量和样品体积无关。在洗涤和计数过程中，标记的蛋白质不会从磁盘上洗脱。C14的绝对计数效率为55%。
Samples (0.02 to 0.12 ml) are removed from a C14 or H3 labeled amino acid incorporating system and applied to 2.3 cm filter paper disks, acid precipitated, washed and extracted batchwise and finally counted directly in a liquid scintillation spectrometer. The count detected is a linear function of the amount of radioactivity added to the disk; it is independent of specific activity, total amount of protein and volume of sample added over a 50-fold change in radioactivity and a 15-fold change in protein concentration. The labeled protein is not eluted from the disk during the washing and counting procedure. The absolute counting efficiency for C14 is 55%.