Composition and antimicrobial resistance profile of Gram‐negative microbiota prevalent in aquacultured fish
Composition and antimicrobial resistance profile of Gram‐negative microbiota prevalent in aquacultured fish
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DOI：
10.1111/jfs.12447
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发表时间：
2018-06
影响因子：
2.4
通讯作者：
M. Ruzauskas;I. Klimienė;J. Armalytė;E. Bartkienė;R. Šiugždinienė;Jūratė Skerniškytė;R. Krasauskas;E. Sužiedėlienė
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作者：
M. Ruzauskas;I. Klimienė;J. Armalytė;E. Bartkienė;R. Šiugždinienė;Jūratė Skerniškytė;R. Krasauskas;E. Sužiedėlienė
水生环境是获得和传播抗微生物药物耐药性的最有利环境之一。本研究的目的有两个：(1)分离和鉴定在开放养殖池塘养殖的鱼类中普遍存在的革兰氏阴性耐药菌；(2)分析其耐药谱。从鱼类中分离出的对至少一种抗菌素具有耐药性的病原菌有119株。最主要的属是假单胞菌、黄杆菌、肠杆菌科和气单胞菌。多重耐药菌株数量最多的是假单胞菌。最低的是aeromonasspp。在所有细菌属中，对-内酰胺类的耐药性是最常见的。对氟喹诺酮类药物的缺乏或极低的耐药率，少量临床重要的耐药基因，以及细菌的分类组成表明，水产养殖鱼类中最广泛的耐药细菌是本地微生物群。实际应用在露天池塘养殖的鱼类中普遍存在的大多数抗微生物细菌可以作为天然（本地微生物群）处理。在这项研究中，在鱼类中检测到的少数耐药细菌可能与来自动物养殖场或人类卫生保健设施的污染水有关。某些菌株，特别是在鱼类中发现的肠杆菌科菌株，含有临床上重要的耐药性决定因素，在食用鱼类加工不当的情况下，这些决定因素可能会传播给人类微生物群。对于耐药菌，除监测鱼类微生物群外，还应采取措施控制养殖池塘入水，并定期调查其耐药性。
Aquatic environments are one of the most favorable settings for acquisition and dissemination of antimicrobial resistance. The aims of this study were twofold: (1) to isolate and identify antimicrobial resistant Gram‐negative bacteria that are prevalent in fish kept in open aquaculture ponds and (2) to analyse their antimicrobial resistance profiles. One hundred and nineteen isolates from fish resistant to at least one antimicrobial were investigated. The most predominant genera werePseudomonas, Chryseobacterium, Enterobacteriaceae, andAeromonas. The highest numbers of multiresistant isolates were found withinPseudomonasspp., and the lowest, inAeromonasspp. Resistance to beta‐lactams was the most common among all of the bacterial genera. The absence or very low rates of resistance to fluoroquinolones, the small number of clinically important resistance genes, and bacterial taxonomic composition suggest that the most widespread resistant bacteria in aquacultured fish are autochthonous microbiota.Practical applicationsThe majority of antimicrobial resistant bacteria that are prevalent in aquacultured fish from open ponds can be treated as natural (autochthonous microbiota). In this study, only a minority of resistant bacteria detected in fish could be associated with polluted waters originating from animal farms or human healthcare facilities. Some strains, particularly the family Enterobacteriaceae that is found in fish, contain clinically important resistance determinants that potentially can be transmitted to human microbiota in cases of inappropriate processing of fish intended for food. With regard to antimicrobial resistant bacteria, measures for control of inflowing waters to aquaculture ponds should be taken in addition to monitoring fish microbiota and its’ resistance should be investigated on a regular basis.