A non-invasive method for in vivo skin volatile compounds sampling
A non-invasive method for in vivo skin volatile compounds sampling
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DOI：
10.1016/j.aca.2013.09.056
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发表时间：
2013-12-04
影响因子：
6.2
通讯作者：
Pawliszyn, Janusz
中科院分区：
文献类型：
--
作者：
Jiang, Ruifen;Cudjoe, Erasmus;Pawliszyn, Janusz
利用人体皮肤散发的挥发性有机化合物（VOCs）对皮肤疾病的诊断具有很大的潜力。这些化合物以非常低的浓度排放。因此，需要在气相色谱-质谱（GC-MS）分析之前执行采样准备步骤。在这项工作中，一个简单的，非侵入性的顶空进样方法，从人体皮肤散发的挥发性化合物，使用薄膜作为萃取相格式。所提出的方法进行了评价的重现性，膜的大小，提取模式和储存条件。首先，小瓶内采样显示膜内和膜间RSD%分别小于9.8%和8.2%，这表明这种自制的皮肤挥发物采样装置在膜内、膜间采样方面具有高度的重现性。体内采样不仅受皮肤代谢状态的影响，而且受环境条件的影响。开发的采样设置（或“膜夹心”）用于比较两种不同的采样模式：顶空和直接采样。结果表明，顶空取样显著降低了背景信号强度，表明来自皮肤表面的污染最小化。此外，对采样前后的膜储存条件进行了充分研究。收集后在干冰中储存长达72 h的膜进行了测试，并显示没有或最小的变化挥发性profiles.This新的皮肤挥发性化合物采样方法结合气相色谱-质谱（GC-MS）可以实现重现性分析。该技术被应用于确定大蒜摄入和酒精摄入的生物标志物。在大蒜摄入过程中检测到二甲基砜、烯丙基甲基硫醚和烯丙基甲基硫醚等代谢产物。此外，从皮肤释放的酒精也被检测到使用我们的“膜三明治”采样。使用相同的方法，我们分析了来自身体上背部、前臂和后大腿区域的皮肤VOC。我们的研究结果表明，不同的身体部位共享一些共同的化合物（27199）。检测到最多化合物的区域是上背部皮肤区域，其中皮脂腺的密度最高。(C)2013爱思唯尔有限公司版权所有。
The use of volatile organic compounds (VOCs) emanating from human skin presents great potential for skin disease diagnosis. These compounds are emitted at very low concentrations. Thus, the sampling preparation step needs to be implemented before gas chromatography-mass spectrometry (GC-MS) analysis. In this work, a simple, non-invasive headspace sampling method for volatile compounds emanating from human skin is presented, using thin film as the extraction phase format.The proposed method was evaluated in terms of reproducibility, membrane size, extraction mode and storage conditions. First, the in vial sampling showed an intra- and inter-membrane RSD% less than 9.8% and 8.2%, respectively, which demonstrated that this home-made skin volatiles sampling device was highly reproducible with regard to intra-, inter-membrane sampling. The in vivo sampling was influenced not only by the skin metabolic status, but also by environmental conditions. The developed sampling setup (or "membrane sandwich") was used to compare two different modes of sampling: headspace and direct sampling. Results demonstrated that headspace sampling had significantly reduced background signal intensity, indicating minimized contamination from the skin surface. In addition, membrane storage conditions both before and after sampling were fully investigated. Membranes stored in dry ice for up to 72 h after collection were tested and showed no or minimal change in volatile profiles.This novel skin volatile compounds sampling approach coupled with gas chromatography-mass spectrometry (GC-MS) can achieve reproducible analysis. This technique was applied to identify the biomarkers of garlic intake and alcohol ingestion. Dimethyl sulphone, allyl methyl sulfide and allyl mercaptan, as metabolites of garlic intake, were detected. In addition, alcohol released from skin was also detected using our "membrane-sandwich" sampling. Using the same approach, we analyzed skin VOCs from upper back, forearm and back thigh regions of the body. Our results show that different body locations share a number of common compounds (27199). The area with most compounds detected was the upper back skin region, where the density of sebaceous glands is the highest. (C) 2013 Elsevier B.V. All rights reserved.