The cellular content of non Hodgkin lymphomas: a comprehensive analysis using monoclonal antibodies and other surface marker techniques.
The cellular content of non Hodgkin lymphomas: a comprehensive analysis using monoclonal antibodies and other surface marker techniques.
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DOI：
10.1038/bjc.1983.52
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发表时间：
1983-03
影响因子：
8.8
通讯作者：
Greaves, M F
中科院分区：
文献类型：
--
作者：
Habeshaw, J A;Bailey, D;Stansfeld, A G;Greaves, M F
采用单克隆抗体α - l - 1、α - l -2a、α - l -3a、OKT1、OKT3、OKT4、OKT6、OKT8、W6/32、26/114、DA-2、2DI、J5、AN51和OKT9，对5例扁桃体、10例反应性淋巴结和65例非霍奇金淋巴瘤（NHL）患者进行悬浮分型，并采用常规的表面标记（E、Fc γ、Fc mu、C3b、C3d）和表面和细胞质免疫球蛋白（SIg、CyIg）重链和轻链分类染色。结果证实了T细胞和T细胞亚群单克隆抗体染色的可重复性和特异性。在一些淋巴瘤（嗜中心细胞、淋巴细胞和免疫母细胞）中，α - l - 1抗体与SIg阳性和Ia阳性细胞有反应性，2例T细胞为主的免疫母细胞淋巴瘤中，OKT3标记SIg阳性细胞。T细胞型淋巴母细胞淋巴瘤表达标记物OKT6。在此基础上提出了诊断T细胞淋巴瘤的标准。单克隆抗体J5，与C-ALL抗原反应，呈可变阳性，偶而在嗜中性和嗜中性滤泡性淋巴瘤的B细胞中呈强阳性。单克隆抗体OKT9染色的细胞比例显示转铁蛋白（trf）受体的细胞表达水平与恶性肿瘤的组织学分级相关，OKT9+细胞在高级别淋巴瘤中升高，在一些低级别组织学分级的转化性淋巴瘤中升高。这些结果进行了讨论，并指出了在NHL表型评估中使用广泛的定义单克隆试剂的优势。
Five samples of tonsil, 10 reactive lymph nodes and 65 consecutive cases of non-Hodgkin lymphoma (NHL) were evaluated in suspension phenotyping with the monoclonal antibodies alpha Leu-I, alpha Leu-2a, alpha Leu-3a, OKT1, OKT3, OKT4, OKT6, OKT8, W6/32, 26/114, DA-2, 2DI, J5, AN51 and OKT9 together with conventional surface marking by rosetting (E, Fc gamma, Fc mu, C3b, C3d) and staining for surface and cytoplasmic immunoglobulin (SIg, CyIg) heavy and light chain classes. The results confirm the reproductability and specificity of staining with monoclonal antibodies against T cells and T cells subsets. Evidence is presented for reactivity of alpha Leu-I antibody with SIg positive and Ia positive cells in some lymphomas (centroblastic centrocytic, lymphocytic and immunoblastic), and 2 cases showed evidence of marking with OKT3 on SIg positive cells in T cell predominant immunoblastic lymphoma. Lymphoblastic lymphomas of T cell type expressed the marker OKT6. On the basis of these results criteria for the diagnosis of T cell lymphoma are suggested. The monoclonal antibody J5, reactive with C-ALL antigen, showed variable positivity, occasionally strong in B cells in cases of centroblastic and centrocytic follicular lymphoma. Proportions of cells staining with the monoclonal antibody OKT9 showed a correlation between levels of cellular expression of transferrin (trf) receptor and the histological grade of malignancy, OKT9+ cells being elevated in high grade lymphomas, and in some cases of transforming lymphoma of low grade histological class. These results are discussed and indicate the advantage of employing a wide range of defined monoclonal reagents in the phenotypic evaluation of NHL.