Antibodies bound to lipid haptens in model membranes diffuse as rapidly as the lipids themselves.
Antibodies bound to lipid haptens in model membranes diffuse as rapidly as the lipids themselves.
复制标题
与模型膜中的脂质半抗原结合的抗体的扩散速度与脂质本身一样快。
DOI：
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发表时间：
1979
影响因子：
11.1
通讯作者：
H. Mcconnell
中科院分区：
文献类型：
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作者：
L. M. Smith;J. W. Parce;B. Smith;H. Mcconnell
图案光漂白方法已用于测量与两个结合位点结合的荧光标记的特异性抗硝基氧 IgG 与脂质体膜中含硝基氧的磷脂的横向扩散速率，所述脂质体膜由 28 ℃ 下的二肉豆蔻酰磷脂酰胆碱（“液体”）、32 ℃ 下的二棕榈酰磷脂酰胆碱（“固体”）和含有 25 ℃ 的二棕榈酰磷脂酰胆碱组成。 15 或 25 mol% 胆固醇（分别为“固体”或“液体”，32 摄氏度）。发现在每种情况下，结合的免疫球蛋白的扩散系数与荧光标记的磷脂的扩散系数相同，尽管这些扩散系数的范围为10(-11)至10(-8)cm2/秒。这里研究的含半抗原的脂质体膜先前已被证明可引发许多抗体依赖性免疫反应。因此，这项工作表明膜结合但自由扩散的抗体足以产生这些反应。
A pattern photobleaching method has been used to measure the rates of lateral diffusion of fluorescent-labeled specific anti-nitroxide IgG bound with both combining sites to nitroxide-containing phospholipids in liposomal membranes composed of dimyristoyl phosphatidylcholine at 28 degrees C ("fluid"), dipalmitoyl phosphatidylcholine at 32 degrees C ("Solid"), and dipalmitoyl phosphatidylcholine containing 15 or 25 mol% cholesterol ("solid" or "fluid," respectively, at 32 degrees C). The diffusion coefficients of the bound immunoglobulin were found to be the same as those of fluorescent-labeled phospholipids in each case even though these diffusion coefficients range from 10(-11) to 10(-8) cm2/sec. Hapten-containing liposomal membranes of the type studied here have previously been shown to elicit a number of antibody-dependent immune responses. Therefore, this work indicates that membrane-bound but otherwise freely diffusing antibodies are sufficient for these reponses.