Fluorescent in situ hybridization on tissue microarrays: challenges and solutions
Fluorescent in situ hybridization on tissue microarrays: challenges and solutions
复制标题
DOI：
10.1007/s10735-006-9069-y
复制
发表时间：
2007-05-01
影响因子：
3.2
通讯作者：
Huntsman, David
中科院分区：
文献类型：
--
作者：
Brown, Lindsay A.;Huntsman, David
组织微阵列（TMA）技术提供了一个高通量的手段，评估潜在的生物标志物和治疗目标的档案病理标本。TMA有助于在一张载玻片上快速评估数百种不同肿瘤的分子变化。来自TMA的切片可用于任何原位组织分析，包括荧光原位杂交（FISH）。FISH是一种检测中期染色体和间期细胞核数量和结构异常的分子技术。FISH通常用作检测易位和评估肿瘤中基因缺失和扩增的预后和诊断工具。对TMA进行FISH使研究人员能够确定数百种高度特征化肿瘤中特定遗传改变的临床意义。在档案石蜡包埋组织上使用FISH在技术上要求很高，并且当应用于石蜡包埋的TMA时变得更具挑战性。本文就FISH技术在TMAs检测中所遇到的问题，包括探针制备、杂交及FISH技术的应用前景等作一综述。
Tissue microarray (TMA) technology has provided a high throughput means of evaluating potential biomarkers and therapeutic targets in archival pathological specimens. TMAs facilitate the rapid assessment of molecular alterations in hundreds of different tumours on a single slide. Sections from TMAs can be used for any in situ tissue analysis, including fluorescent in situ hybridization (FISH). FISH is a molecular technique that detects numerical and structural abnormalities in both metaphase chromosomes and interphase nuclei. FISH is commonly used as a prognostic and diagnostic tool for the detection of translocations and for the assessment of gene deletion and amplification in tumours. Performing FISH on TMAs enables researchers to determine the clinical significance of specific genetic alterations in hundreds of highly characterized tumours. The use of FISH on archival paraffin embedded tissues is technically demanding and becomes even more challenging when applied to paraffin embedded TMAs. The problems encountered with FISH on TMAs, including probe preparation, hybridization, and potential applications of FISH, will be addressed in this review.