MICROTUBULES AND THEIR ORGANIZING CENTERS IN DIFFERENTIATING GUARD-CELLS OF ADIANTUM-CAPILLUS-VENERIS
MICROTUBULES AND THEIR ORGANIZING CENTERS IN DIFFERENTIATING GUARD-CELLS OF ADIANTUM-CAPILLUS-VENERIS
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DOI：
10.1007/bf01279808
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发表时间：
1983-01-01
期刊：
影响因子：
2.9
通讯作者：
KATSAROS, C
中科院分区：
文献类型：
--
作者：
GALATIS, B;APOSTOLAKOS, P;KATSAROS, C
幼保卫细胞的皮层细胞质;阴茎毛局部分化。在早期后期阶段，许多微管从细胞质中分叉，占据腹面壁与平周壁的中间区域的交界处，朝向平周壁和腹面壁面，并朝向内部细胞质。微管囊泡复合物（MVC）检测在这些地区。它们的出现伴随着相同区域局部壁增厚的开始。之后，在周壁的皮质细胞质中观察到锚定到质膜上的更明显的MVC，靠近生长的增厚，通常距离它们最多3 μ m。有时它们似乎含有电子致密物质，微管嵌入其中。皮层微管从一个以上的方向会聚终止于MCV。排列在平周壁上的微管群从上述细胞质形成的区域向外辐射。上覆的纤维素微纤丝表现出相同的取向。位于MCV的囊泡显然是网质体来源的，电子密度很高，对过碘酸-硫代碳酰肼-银蛋白（PA-TCH-SP）试验呈阳性反应。另一群微管从MCV呈扇形散开，进入细胞质更深处。它们与细胞核和线粒体相连。它们与细胞核和线粒体结合，并穿过周核小体的细胞质。在某些情况下，核形成了一个突出的MVC，并出现与它通过微管辐射从MVC和侧翼的核膜。突出微管组织中心（MTOC）的功能在皮层细胞质的中间区域的围壁周围的腹侧一个相对较长的时间。MVC和/或其相邻的质膜位点可能代表MTOC或至少它们指定微管成核的皮质细胞质位点。
The cortical cytoplasm of the young guard cells of A. capillus veneris is locally differentiated. At an early post-telophase stage, numerous microtubules diverge from the cytoplasm occupying the junctions of the midregion of the ventral wall with the periclinal ones, towards the periclinal and ventral wall faces, and towards the inner cytoplasm. Microtubule-vesicle complexes (MVC) are detected in these regions. Their appearance is accompanied by the initiation of local wall thickenings in the same areas. Afterwards, more distinct MVC anchored to the plasmalemma were seen in the cortical cytoplasm of the periclinal walls, close to the growing thickenings, usually at a distance up to 3 .mu.m from them. Sometimes they seemed to contain an electron dense substance in which the microtubules were embedded. Cortical microtubules converging from more than 1 direction terminate at the MCV. The microtubule population lining the periclinal walls radiate from the regions where the above cytoplasmic formations are localized. The overlying cellulose microfibrils exhibit the same orientation. The vesicles localized at the MCV apparently are of dictyosomal origin, very electron dense and react positively to periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) test. Another population of micotubules fan out from the MCV, entering deeper into the cytoplasm. They become associated with the nucleus and mitochondria. They become associated with the nucleus and mitochondria, and traverse the peridictyosomal cytoplasm. In some instances the nucleus formed a protrusion towards an MVC and appeared associated with it via microtubules which radiate from the MVC and flank the nuclear envelope. Prominent microtubule organizing centers (MTOC) function in the cortical cytoplasm of the midregion of the periclinal walls surrounding the ventral one for a relatively long time. The MVC and/or their adjacent plasmalemma sites may represent MTOC or at least they specify the cortical cytoplasmic sites where microtubules are nucleated.