[Microscopic diagnosis of onychomycoses].
[Microscopic diagnosis of onychomycoses].
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甲真菌病的显微诊断[J].
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
C. Piérard‐franchimont
中科院分区：
文献类型：
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作者：
G. Piérard;J. Arrese;S. Pierre;C. Bertrand;P. Corcuff;J. Lévêque;C. Piérard‐franchimont
介绍
甲真菌病的微生物鉴定可能不确定，因为从该材料培养的一些真菌不一定是病原体和侵入性的。相反，即使通过显微镜观察到真菌，培养物的阴性也并不罕见。
材料和方法
我们比较了标准组织学检查，免疫组织化学和体内共聚焦显微镜所带来的信息。
结果
指甲的组织学检查在这种病理学中是重要的。我们提出了一种实验室技术，证明是简单和快速的。我们报告的诊断标准允许皮肤真菌，酵母菌和非皮肤真菌霉菌之间的区别。标准的显微镜检查可进一步改进免疫组化使用一些抗体的真菌。活体共聚焦显微镜是未来的技术。皮肤科医生将能够在临床检查时看到指甲中的真菌，而无需任何取样或特殊准备。
结论
组织学检查是一种常规技术，可用于确定指甲盖中真菌的性质和定位。免疫组化应用于甲真菌病是一种实验方法，带来了有关真菌鉴定的重要信息。活体共聚焦显微镜是一扇通向未来的大门。
INTRODUCTION
The microbiological identification of onychomycosis may be uncertain as some fungi growing in culture from this material are not necessarily pathogen and invasive. Conversely, the negativity of a culture is not rare even when fungi are seen by microscopy.
MATERIALS AND METHODS
We compared the information brought by standard histological examination, immunohistochemistry and in vivo confocal microscopy.
RESULTS
The histological examination of nails is of importance in this pathology. We present a laboratory technique that proves to be easy and rapid. We report diagnostic criteria allowing the distinction between dermatophytes, yeasts and non-dermatophyte molds. The standard microscopic examination may further be improved by immunohistochemistry using some antibodies to fungi. In vivo confocal microscopy is a technique for the future. The dermatologist will be able to see fungi in the nail at the clinical examination, without any sampling or peculiar preparation.
CONCLUSION
The histological examination is a routine technique useful for defining the nature and localization of fungi in the nail plate. Immunohistochemistry applied to onychomycosis is an experimental approach bringing prominent informations about the identification of fungi. In vivo confocal microscopy is a door opened to the future.