Expression of periostin in the serum of NSCLC and its function on proliferation and migration of human lung adenocarcinoma cell line (A549) in vitro
Expression of periostin in the serum of NSCLC and its function on proliferation and migration of human lung adenocarcinoma cell line (A549) in vitro
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DOI：
10.1007/s11033-009-9721-1
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发表时间：
2010-06-01
影响因子：
2.8
通讯作者：
Shi, Yi
中科院分区：
文献类型：
--
作者：
Hong, Lingzhi;Sun, Huiming;Shi, Yi
骨膜蛋白在许多上皮性恶性肿瘤中过表达，包括肺癌、乳腺癌、卵巢癌和结肠癌。它与乳腺癌和卵巢癌细胞在体外的生长和迁移有关。本研究的目的是通过酶联免疫吸附血清学方法研究非小细胞肺癌（NSCLC）患者血清骨膜蛋白水平及其与生物学和预后因素的关系。观察骨膜蛋白对人肺腺癌细胞株A549增殖和迁移的影响，并探讨其作用机制。与正常健康志愿者（215.66 +/- A 11.67 pg/ml）相比，NSCLC患者（242.84 +/- A 5.33 pg/ml）的血清骨膜蛋白（POSTs）平均值升高（p = 0.030）。血清骨膜蛋白水平与NSCLC患者的性别、年龄、病理类型、TNM分期、淋巴结状况、肿瘤大小及侵袭性无关。我们构建了一个表达人骨膜蛋白全长的质粒pEGFP-N1/pEGFP-N1。将该质粒转染A549细胞，骨膜蛋白在转染细胞中得到高效表达。结果表明，periostin可通过诱导vimentin和N-cadherin的表达，下调E-cadherin的表达，促进A549细胞的增殖和迁移。这些结果有力地表明，骨膜蛋白是一种新的分子，在非小细胞肺癌的进展和发展中发挥重要作用。
Periostin is over expressed in many epithelial malignant cancers, including lung cancer, breast cancer, ovarian cancer and colon cancer. It is related with the progression and migration of breast and ovarian cancer cells in vitro. The aim of this study was to investigate the serum level of periostin in non-small cell lung cancer (NSCLC) and its relationship with established biological and prognostic factors by enzyme-linked-immunosorbent serologic assay. We also observe the function of periostin on the proliferation and migration of human lung adenocarcinoma cell line (A549) and discuss the mechanism. The mean value for serum periostin (POSTN) was elevated in NSCLC patients (242.84 +/- A 5.33 pg/ml) compared to the normal healthy volunteers (215.66 +/- A 11.67 pg/ml) (p = 0.030). The serum level of periostin of NSCLC patients had no connection with gender, age, pathological type, TNM stage, lymph node status, tumor size and invasiveness. We constructed a plasmid named pEGFP-N1/POSTN expressing full-length human periostin. Transfecting the plasmid to A549 cells and periostin was efficiently expressed in transfected A549 cells. Our data showed that periostin could promote the proliferation and migration of A549 cells by inducing vimentin and N-cadherin expression and downregulating E-cadherin expression. These results strongly suggest that periostin is a novel molecular which play an important role during the progression and development of NSCLC.