Plasmid-only rescue of influenza A virus vaccine candidates
Plasmid-only rescue of influenza A virus vaccine candidates
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DOI：
10.1098/rstb.2001.0979
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发表时间：
2001-12-29
期刊：
影响因子：
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通讯作者：
Palese, P
中科院分区：
文献类型：
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作者：
Schickli, JH;Flandorfer, A;Palese, P
另一场流感病毒大流行的潜在威胁促使人们讨论如何为这一事件做好准备。最合理的预防方法似乎是使用有效的疫苗。由于流感病毒和其他负链RNA病毒可通过质粒转染进行基因操作，因此有可能概述基于反向遗传学的流感病毒疫苗接种新方法。我们建议采取三种方法。首先，我们使用了一种质粒拯救系统，可以快速产生高产的重组疫苗菌株。其次，我们建议通过构建具有干扰素拮抗剂作用的NSI蛋白缺失的减毒主株来开发第二代流感病毒活疫苗。第三，我们建议使用表达流感病毒血凝素蛋白的新城疫病毒重组体作为新型疫苗载体，用于动物和可能的人类。
The potential threat of another influenza virus pandemic stimulates discussion on how to prepare for such an event. The most reasonable prophylactic approach appears to be the use of effective vaccines. Since influenza and other negative-stranded RNA viruses are amenable to genetic manipulation using transfection by plasmids, it is possible to outline new reverse genetics-based approaches for vaccination against influenza viruses. We suggest three approaches. First, we use a plasmid-only rescue system that allows the rapid generation of high-yield recombinant vaccine strains. Second, we propose developing second-generation live influenza virus vaccines by constructing an attenuated master strain with deletions in the NSI protein, which acts as an interferon antagonist. Third, we suggest the use of Newcastle disease virus recombinants expressing influenza virus haemagglutinin proteins of pandemic (epizootic) strains as novel vaccine vectors for use in animals and possibly humans.