MULTIPLE EXTRACELLULAR ACTIVITIES IN DROSOPHILA EGG PERIVITELLINE FLUID ARE REQUIRED FOR ESTABLISHMENT OF EMBRYONIC DORSAL-VENTRAL POLARITY
MULTIPLE EXTRACELLULAR ACTIVITIES IN DROSOPHILA EGG PERIVITELLINE FLUID ARE REQUIRED FOR ESTABLISHMENT OF EMBRYONIC DORSAL-VENTRAL POLARITY
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DOI：
10.1016/0092-8674(92)90181-b
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发表时间：
1992-02-07
期刊：
影响因子：
64.5
通讯作者：
NUSSLEINVOLHARD, C
中科院分区：
文献类型：
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作者：
STEIN, D;NUSSLEINVOLHARD, C
在果蝇胚胎中，12个母体效应基因(背部基因和仙人掌基因)是建立胚胎背腹轴所必需的。胚胎的背腹两极是由Toll受体配体的腹侧形成在胚胎周围的卵黄周室内定义的。在这里，通过移植卵周液，我们证明了在卵周液中存在三种不同的活性，它们可以分别恢复突变的复活节、蛇和铲形胚胎的背腹极性。这些活动不能定义背腹轴的极性；相反，它们根据突变胚胎固有的背腹轴极性恢复结构。它们似乎参与了Toll蛋白的腹侧配体的形成。这一过程需要丝氨酸蛋白水解酶活性；丝氨酸蛋白酶抑制剂感染野生型胚胎的卵周隙会导致背化胚胎的形成。
Twelve maternal effect genes (the dorsal group and cactus) are required for the establishment of the embryonic dorsal-ventral axis in the Drosophila embryo. Embryonic dorsal-ventral polarity is defined within the perivitelline compartment surrounding the embryo by the ventral formation of a ligand for the Toll receptor. Here, by transplantation of perivitelline fluid we demonstrate the presence of three separate activities present in the perivitelline fluid that can restore dorsal-ventral polarity to mutant easter, snake, and spatzle embryos, respectively. These activities are not capable of defining the polarity of the dorsal-ventral axis; instead they restore structures according to the intrinsic dorsal-ventral polarity of the mutant embryos. They appear to be involved in the ventral formation of a ligand for the Toll protein. This process requires serine proteolytic activity; the infection of serine protease inhibitors into the perivitelline space of wild-type embryos results in the formation of dorsalized embryos.