RELATIONSHIP OF MOUSE MINOR SATELLITE DNA TO CENTROMERE ACTIVITY
RELATIONSHIP OF MOUSE MINOR SATELLITE DNA TO CENTROMERE ACTIVITY
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DOI：
10.1159/000132989
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发表时间：
1990-01-01
期刊：
影响因子：
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通讯作者：
MILLER, DA
中科院分区：
文献类型：
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作者：
BROCCOLI, D;MILLER, OJ;MILLER, DA
用H-3标记的小鼠小卫星(卫星II)DNA进行原位杂交前，用Q显带方法鉴定了一个雌性小鼠细胞系的染色体。没有发现标记每条染色体的细胞，但颗粒计数表明，每个活跃的着丝粒区域都有微小的卫星序列。在小鼠T(10；13)199H易位中，断裂点在小卫星阵列内，在13(10)染色体的无C带活性着丝粒和10(13)染色体的间隙C带上留下了小卫星簇，这与着丝粒活性无关。在一条带有一端和两条间隙C带的小鼠A9(L细胞衍生)标记染色体中，只有末端的C带与活性着丝粒相邻，但在所有三个位置上都存在小卫星DNA。在Y染色体上没有检测到小卫星DNA，但不能排除该染色体上存在少量发散的卫星序列。
Chromosomes from a female mouse cell line were identified by Q-banding prior to in situ hybridization with H-3-labeled mouse minor satellite (satellite II) DNA. No cell was found in which every chromosome was labeled, but grain counts showed that every active centromeric region had minor satellite sequences. In the mouse T(10;13)199H translocation, the breakpoint was within the minor satellite array, leaving clusters of minor satellite at the C-bandless active centromere of the 13(10) chromosome and at the interstitial C-band of the 10(13) chromosome, which is not associated with centromeric activity. In a mouse A9 (L-cell derived) marker chromosome with one terminal and two interstitial C-bands, only the terminal C-band was adjacent to an active centromere, but minor satellite DNA was present at all three sites. Minor satellite DNA was not detected on the Y chromosome, although the presence of a small amount of divergent satellite sequences on this chromosome could not be ruled out.