Elevated levels of periostin and TGF-β1 in the bronchoalveolar lavage fluid of patients with idiopathic eosinophilic pneumonia
Elevated levels of periostin and TGF-β1 in the bronchoalveolar lavage fluid of patients with idiopathic eosinophilic pneumonia
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DOI：
10.12932/ap-111018-0414
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发表时间：
2020-09-01
影响因子：
5
通讯作者：
Kobashi, Yoshihiro
中科院分区：
文献类型：
--
作者：
Katoh, Shigeki;Matsumoto, Nobuhiro;Kobashi, Yoshihiro
背景资料：骨膜蛋白在支气管上皮细胞和成纤维细胞中由包括白细胞介素（IL）-13和转化生长因子（TGF）-β（1）在内的各种刺激物诱导，并且参与过敏性疾病如哮喘和特应性皮炎，在组织重塑和纤维化中起重要作用。目的：为了检测骨膜蛋白对人类嗜酸性肺炎症的作用，我们评估了嗜酸性肺炎（EP）患者支气管肺泡灌洗液（BALF）中骨膜蛋白、IL-13和TGF-β（1）的水平。方法：采用酶联免疫吸附试验测定急性EP、慢性EP、特发性肺纤维化（IPF）和结节病患者BALF中骨膜蛋白、IL-13和TGF-β 1的浓度。结果：EP患者BALF中嗜酸性粒细胞绝对数量、骨膜蛋白、IL-13和TGF-β 1水平均显著高于IPF和结节病患者。在EP患者的BALF中，骨膜蛋白的浓度与TGF-β（1）的浓度显著相关，但与IL-13的浓度无关。骨膜蛋白水平也与IPF患者BALF中嗜酸性粒细胞的绝对数量显著相关，但与EP无关。结论：我们的研究结果表明，TGF-β（1）可能会增加EP患者肺部骨膜蛋白的产生。骨膜蛋白不仅参与了EP的发病机制，而且参与了IPE的发病机制。
Background: Periostin is induced in bronchial epithelial cells and fibroblasts by various stimuli including interleukin (IL)-13 and transforming growth factor (TGF)-beta(1), and is involved in allergic diseases such as asthma and atopic dermatitis, playing an important role in tissue remodeling and fibrosis. The role of periostin in the pathogenesis of cosinophilic lung diseases, however, is unclear.Objective: To examine the contribution of periostin to cosinophilic inflammation of the lung in humans, we evaluated periostin, IL-13, and TGF-beta(1) levels in the bronchoalveolar lavage fluid (BALF) of patients with cosinophilic pneumonia (EP).Methods: Periostin, IL-13, and TGF-beta(1) concentrations in the BALF were measured by enzyme-linked immunosorbent assay in patients with acute EP, chronic EP, idiopathic pulmonary fibrosis (IPF), and sarcoidosis. Further, we analyzed the relationship between periostin, IL-13, and TGF-beta(1), levels and the number of inflammatory cells in the BALE.Results: The absolute number ofeosinophils, and the periostin, IL-13, and TGF-beta(1) levels in the BALF were significantly higher in patients with EP than in patients with IPF and sarcoidosis. Concentrations of periostin significantly correlated with the concentrations of TGF-beta(1), but not those of IL-13, in the BALF of patients with EP. Periostin levels also significantly correlated with the absolute number of cosinophils in the BALF of patients with IPF, but not EP.Conclusions: Our findings suggest that TGF-beta(1) might increase the production of periostin in the lungs of patients with EP. Periostin might contribute the pathogenesis of not only EP, but also IPE.