Identification of miR-125a-5p as a tumor suppressor of renal cell carcinoma, regulating cellular proliferation, migration and apoptosis
Identification of miR-125a-5p as a tumor suppressor of renal cell carcinoma, regulating cellular proliferation, migration and apoptosis
复制标题
鉴定 miR-125a-5p 作为肾细胞癌的肿瘤抑制因子，调节细胞增殖、迁移和凋亡
DOI：
10.3892/mmr.2014.2848
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发表时间：
2015-02-01
影响因子：
3.4
通讯作者：
Lai, Yongqing
中科院分区：
文献类型：
--
作者：
Chen, Duqun;Li, Yifan;Lai, Yongqing
miR-125 a-5 p在许多恶性肿瘤中被描述为肿瘤抑制因子，然而miR-125 a-5 p在肾细胞癌（RCC）中的表达和功能仍有待阐明。为了探讨miR-125 a-5 p在肾细胞癌中的潜在作用，本研究采用定量聚合酶链反应（PCR）检测了肾癌组织中miR-125 a-5 p的表达水平。还分别使用MTT测定、伤口划痕测定和流式细胞术测定miR-125 a-5 p对细胞增殖、迁移和凋亡的影响。miR-125 a-5 p的表达在RCC中显示降低，并且通过合成模拟物恢复miR-125 a-5 p显示抑制细胞增殖和迁移，并诱导凋亡。目前的结果表明，miR-125 a-5 p可能在RCC中起肿瘤抑制剂的作用。据我们所知，本研究首次证明了miR-125 a-5 p在RCC中的下调，并显示了它在影响细胞增殖，迁移和凋亡中的作用。需要进一步研究确定miR-125 a-5 p的靶基因，并探索miR-125 a-5 p作为RCC诊断或预后生物标志物的潜力。
miR-125a-5p has been previously described as a tumor suppressor in numerous malignancies, however the expression and function of miR-125a-5p in renal cell carcinoma (RCC) remains to be elucidated. In the present study, to explore the potential role of miR-125a-5p in RCC, quantitative polymerase chain reaction was used to determine the expression levels of miR-125a-5p in renal cancer tissues. The influence of miR-125a-5p on cell proliferation, migration and apoptosis was also determined, using an MTT assay, a wound scratch assay and flow cytometry, respectively. The expression of miR-125a-5p was shown to be decreased in RCC and the restoration of miR-125a-5p by synthetic mimics was shown to suppress cell proliferation and migration, and induce apoptosis. The present results indicate that miR-125a-5p may function as a tumor suppressor in RCC. The present study is, to the best of our knowledge, the first to demonstrate the downregulation of miR-125a-5p in RCC, and to show the role it has in affecting cellular proliferation, migration and apoptosis. Further research is needed to define the target genes of miR-125a-5p and explore the potential of miR-125a-5p as a diagnostic or a prognostic biomarker for RCC.